PMID- 24941918
OWN - NLM
STAT- MEDLINE
DCOM- 20141103
LR  - 20191210
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 164
IP  - 2
DP  - 2014
TI  - Pomegranate ( Punica granatum L.) expresses several nsLTP isoforms characterized 
      by different immunoglobulin E-binding properties.
PG  - 112-21
LID - 10.1159/000362761 [doi]
AB  - BACKGROUND: Pomegranate allergy is associated with sensitization to non-specific 
      lipid transfer proteins (nsLTPs). Our aim was to identify and characterize the 
      non-specific nsLTPs expressed in pomegranate at the molecular level and to study 
      their allergenic properties in terms of immunoglobulin E (IgE)-binding and 
      cross-reactivity with peach nsLTP (Pru p 3). METHODS: A non-equilibrium 
      two-dimensional (2-D) electrophoretic approach based on acid-urea PAGE and sodium 
      dodecyl sulfate PAGE was set up to separate pomegranate nsLTPs. Their 
      immunoreactivity was tested by immunoblotting carried out with anti-Pru p 3 
      polyclonal antibodies and sera from pomegranate-allergic patients. For final 
      identification, pomegranate nsLTPs were purified by chromatography and subjected 
      to trypsin digestion and mass spectrometry (MS) analysis. For this purpose, the 
      sequences obtained by cDNA cloning of three pomegranate nsLTPs were integrated in 
      the database that was subsequently searched for MS data interpretation. RESULTS: 
      Four nsLTPs were identified by 2-D immunoblotting. The detected proteins showed 
      different IgE-binding capacity and partial cross-reactivity with Pru p 3. cDNA 
      cloning and MS analyses led to the identification of three nsLTP isoforms with 
      66-68% amino acid sequence identity named Pun g 1.0101, Pun g 1.0201 and Pun g 
      1.0301. CONCLUSIONS: By 2-D electrophoresis, we could separate different nsLTP 
      isoforms possessing different IgE-binding properties, which might reflect 
      peculiar allergenic potencies. The contribution of Pru p 3 to prime sensitization 
      is not central as in other plant nsLTPs.
CI  - (c) 2014 S. Karger AG, Basel.
FAU - Bolla, Michela
AU  - Bolla M
AD  - Department of Biotechnology, University of Verona, Verona, Italy.
FAU - Zenoni, Sara
AU  - Zenoni S
FAU - Scheurer, Stephan
AU  - Scheurer S
FAU - Vieths, Stefan
AU  - Vieths S
FAU - San Miguel Moncin, Maria Del Mar
AU  - San Miguel Moncin Mdel M
FAU - Olivieri, Mario
AU  - Olivieri M
FAU - Antico, Andrea
AU  - Antico A
FAU - Ferrer, Marta
AU  - Ferrer M
FAU - Berroa, Felicia
AU  - Berroa F
FAU - Enrique, Ernesto
AU  - Enrique E
FAU - Avesani, Linda
AU  - Avesani L
FAU - Marsano, Francesco
AU  - Marsano F
FAU - Zoccatelli, Gianni
AU  - Zoccatelli G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140617
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Plant Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (sterol carrier proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
CIN - Int Arch Allergy Immunol. 2014;164(3):169-70. PMID: 25011525
MH  - Adult
MH  - Allergens/genetics/immunology
MH  - Amino Acid Sequence
MH  - Antigens, Plant/immunology
MH  - Carrier Proteins/*genetics/*immunology
MH  - Cross Reactions/immunology
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Food Hypersensitivity/genetics/immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Lythraceae/genetics/*immunology
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Plant Proteins/genetics/immunology
MH  - Protein Isoforms/*genetics/*immunology
MH  - Prunus/genetics/immunology
MH  - Sequence Alignment
MH  - Young Adult
EDAT- 2014/06/20 06:00
MHDA- 2014/11/05 06:00
CRDT- 2014/06/20 06:00
PHST- 2013/11/08 00:00 [received]
PHST- 2014/04/09 00:00 [accepted]
PHST- 2014/06/20 06:00 [entrez]
PHST- 2014/06/20 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
AID - 000362761 [pii]
AID - 10.1159/000362761 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2014;164(2):112-21. doi: 10.1159/000362761. Epub 2014 
      Jun 17.