PMID- 24942351
OWN - NLM
STAT- MEDLINE
DCOM- 20150224
LR  - 20140709
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Linking)
VI  - 25
IP  - 12
DP  - 2014 Aug 20
TI  - In-situ administration of dendritic cells following argon-helium cryosurgery 
      enhances specific antiglioma immunity in mice.
PG  - 900-8
LID - 10.1097/WNR.0000000000000196 [doi]
AB  - Dendritic cells (DCs) are highly specialized antigen-presenting cells that play a 
      key role in the activation of naive T cells. With an aim to explore whether 
      in-situ administration of DCs following argon-helium cryosurgery could enhance 
      specific antiglioma immunity in mice, we evaluated the validity of this approach 
      in a murine subcutaneous GL261 glioma model. C57BL/6 mice models bearing 
      subcutaneous GL261 glioma were established and then divided into four groups, 
      namely, no-treatment group (n=14), DC group (n=14), cryosurgery group (n=15), and 
      cryosurgery+DC group (n=15). Compared with the other groups, cryosurgery combined 
      with DCs injection reduced tumor sizes and significantly prolonged survival. In 
      addition, the combined treatment resulted in significantly increasing percentages 
      of CD3, CD3CD4 cells, the ratio of CD3CD4/CD3CD8, and the level of serum 
      interleukin-12 10 days after treatments. Furthermore, in the combined treatment 
      group, Th1 cells were significantly higher than those in the other groups, and 
      the splenic cytotoxic T lymphocyte of mice showed significantly increasing 
      specific cytotoxicity against GL261 cells. These results indicated that in 
      addition to the destruction of tumor, cryosurgery combined with DCs injection 
      enhanced systemic antitumor immunity, suggesting the potential usefulness of the 
      combined treatment in the clinical management of gliomas.
FAU - Lin, Chunnan
AU  - Lin C
AD  - aDepartment of Neurosurgery, Zhujiang Hospital bInstitute of Neurosurgery, Key 
      Laboratory on Brain Function Repair and Regeneration of Guangdong, Southern 
      Medical University, Guangzhou, China cDepartment of Neurosurgery, The First 
      Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
FAU - Wang, Qifu
AU  - Wang Q
FAU - Lu, Guohui
AU  - Lu G
FAU - Yin, Zhilin
AU  - Yin Z
FAU - He, Xiaozheng
AU  - He X
FAU - Xu, Hongchao
AU  - Xu H
FAU - Pan, Jun
AU  - Pan J
FAU - Zhang, Shizhong
AU  - Zhang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 187348-17-0 (Interleukin-12)
RN  - 206GF3GB41 (Helium)
RN  - 67XQY1V3KH (Argon)
SB  - IM
MH  - Animals
MH  - Argon
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Combined Modality Therapy
MH  - Cryosurgery/*methods
MH  - Dendritic Cells/*transplantation
MH  - Disease Models, Animal
MH  - Female
MH  - Glioma/*immunology/pathology/*therapy
MH  - Helium
MH  - Interleukin-12/blood
MH  - Mice, Inbred C57BL
MH  - Neoplasm Transplantation
MH  - Random Allocation
MH  - Spleen/immunology/pathology
MH  - Survival Analysis
MH  - T-Lymphocytes/immunology
EDAT- 2014/06/20 06:00
MHDA- 2015/02/25 06:00
CRDT- 2014/06/20 06:00
PHST- 2014/06/20 06:00 [entrez]
PHST- 2014/06/20 06:00 [pubmed]
PHST- 2015/02/25 06:00 [medline]
AID - 10.1097/WNR.0000000000000196 [doi]
PST - ppublish
SO  - Neuroreport. 2014 Aug 20;25(12):900-8. doi: 10.1097/WNR.0000000000000196.