PMID- 24945110 OWN - NLM STAT- MEDLINE DCOM- 20150409 LR - 20140816 IS - 1878-5867 (Electronic) IS - 0039-128X (Linking) VI - 88 DP - 2014 Oct TI - Regional specific regulation of steroid receptor coactivator-1 immunoreactivity by orchidectomy in the brain of adult male mice. PG - 7-14 LID - S0039-128X(14)00145-7 [pii] LID - 10.1016/j.steroids.2014.06.006 [doi] AB - Androgens including testosterone and dihydrotestosterone play important roles on brain structure and function, either directly through androgen receptor or indirectly through estrogen receptors, which need coactivators for their transcription activation. Steroid receptor coactivator-1 (SRC-1) has been shown to be multifunctional potentials in the brain, but how it is regulated by androgens in the brain remains unclear. In this study, we explored the effect of orchidectomy (ORX) on the expression of SRC-1 in the adult male mice using nickel-intensified immunohistochemistry. The results showed that ORX induced dramatic decrease of SRC-1 immunoreactivity in the olfactory tubercle, piriform cortex, ventral pallidum, most parts of the septal area, hippocampus, substantia nigra (compact part), pontine nuclei and nucleus of the trapezoid body (p<0.01). Significant decrease of SRC-1 was noticed in the dorsal and lateral septal nucleus, medial preoptical area, dorsomedial and ventromedial hypothalamic nucleus and superior paraolivary nucleus (p<0.05). Whereas in other regions examined, levels of SRC-1 immunoreactivity were not obviously changed by ORX (p>0.05). The above results demonstrated ORX downregulation of SRC-1 in specific regions that have been involved in sense of smell, learning and memory, cognition, neuroendocrine, reproduction and motor control, indicating that SRC-1 play pivotal role in the mediating circulating androgenic regulation on these important brain functions. It also indicates that SRC-1 may serve as a novel target for the central disorders caused by the age-related decrease of circulating androgens. CI - Copyright (c) 2014 Elsevier Inc. All rights reserved. FAU - Bian, Chen AU - Bian C AD - Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China. FAU - Zhang, Kaiyuan AU - Zhang K AD - Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China; Cadet Brigade, Third Military Medical University, Chongqing 400038, China. FAU - Zhao, Yangang AU - Zhao Y AD - Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China. FAU - Guo, Qiang AU - Guo Q AD - Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China. FAU - Cai, Wenqin AU - Cai W AD - Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China. Electronic address: caiwenqin@tmmu.edu.cn. FAU - Zhang, Jiqiang AU - Zhang J AD - Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China. Electronic address: zhangjqtmmu@yahoo.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140616 PL - United States TA - Steroids JT - Steroids JID - 0404536 RN - 0 (Androgens) RN - EC 2.3.1.48 (Nuclear Receptor Coactivator 1) SB - IM MH - Androgens/deficiency MH - Animals MH - Brain/*metabolism MH - Gene Expression Regulation MH - Immunohistochemistry MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Nuclear Receptor Coactivator 1/*metabolism MH - *Orchiectomy OTO - NOTNLM OT - Androgen OT - Nuclear receptor OT - Orchidectomy OT - Sex hormone EDAT- 2014/06/20 06:00 MHDA- 2015/04/10 06:00 CRDT- 2014/06/20 06:00 PHST- 2014/02/26 00:00 [received] PHST- 2014/05/13 00:00 [revised] PHST- 2014/06/04 00:00 [accepted] PHST- 2014/06/20 06:00 [entrez] PHST- 2014/06/20 06:00 [pubmed] PHST- 2015/04/10 06:00 [medline] AID - S0039-128X(14)00145-7 [pii] AID - 10.1016/j.steroids.2014.06.006 [doi] PST - ppublish SO - Steroids. 2014 Oct;88:7-14. doi: 10.1016/j.steroids.2014.06.006. Epub 2014 Jun 16.