PMID- 24945745
OWN - NLM
STAT- MEDLINE
DCOM- 20151013
LR  - 20240322
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 6
DP  - 2014
TI  - Long-distance communication between laryngeal carcinoma cells.
PG  - e99196
LID - 10.1371/journal.pone.0099196 [doi]
LID - e99196
AB  - Tunneling nanotubes and epithelial bridges are recently discovered new forms of 
      intercellular communication between remote cells allowing their electrical 
      synchronization, transfer of second messengers and even membrane vesicles and 
      organelles. In the present study, we demonstrate for the first time in primary 
      cell cultures prepared from human laryngeal squamous cell carcinoma (LSCC) 
      samples that these cells communicate with each other over long distances (up to 1 
      mm) through membranous tunneling tubes (TTs), which can be open-ended or contain 
      functional gap junctions formed of connexin 43. We found two types of TTs, 
      containing F-actin alone or F-actin and alpha-tubulin. In the LSCC cell culture, we 
      identified 5 modes of TT formation and performed quantitative assessment of their 
      electrical properties and permeability to fluorescent dyes of different molecular 
      weight and charge. We show that TTs, containing F-actin and alpha-tubulin, transport 
      mitochondria and accommodate small DAPI-positive vesicles suggesting possible 
      transfer of genetic material through TTs. We confirmed this possibility by 
      demonstrating that even TTs, containing gap junctions, were capable of 
      transmitting double-stranded small interfering RNA. To support the idea that the 
      phenomenon of TTs is not only typical of cell cultures, we have examined 
      microsections of samples obtained from human LSCC tissues and identified 
      intercellular structures similar to those found in the primary LSCC cell culture.
FAU - Antanaviciute, Ieva
AU  - Antanaviciute I
AD  - Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, 
      Lithuania.
FAU - Rysevaite, Kristina
AU  - Rysevaite K
AD  - Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, 
      Lithuania; Institute of Anatomy, Lithuanian University of Health Sciences, 
      Kaunas, Lithuania.
FAU - Liutkevicius, Vykintas
AU  - Liutkevicius V
AD  - Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, 
      Lithuania; Department of Otorhinolaryngology, Lithuanian University of Health 
      Sciences, Kaunas, Lithuania.
FAU - Marandykina, Alina
AU  - Marandykina A
AD  - Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, 
      Lithuania.
FAU - Rimkute, Lina
AU  - Rimkute L
AD  - Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, 
      Lithuania.
FAU - Sveikatiene, Renata
AU  - Sveikatiene R
AD  - Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, 
      Lithuania.
FAU - Uloza, Virgilijus
AU  - Uloza V
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      Kaunas, Lithuania.
FAU - Skeberdis, Vytenis Arvydas
AU  - Skeberdis VA
AD  - Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, 
      Lithuania.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140619
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Actins)
RN  - 0 (Connexin 43)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (LST1 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Actins/metabolism
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/pathology
MH  - Cell Communication/*genetics
MH  - Connexin 43/*metabolism
MH  - Fluorescent Dyes
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Membrane Proteins/metabolism
MH  - Microtubules/metabolism
MH  - Mitochondria/metabolism/pathology
MH  - Nanotubes/chemistry
MH  - Optical Imaging
MH  - Permeability
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Tissue Culture Techniques
PMC - PMC4063716
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/06/20 06:00
MHDA- 2015/10/16 06:00
PMCR- 2014/06/19
CRDT- 2014/06/20 06:00
PHST- 2014/02/17 00:00 [received]
PHST- 2014/05/12 00:00 [accepted]
PHST- 2014/06/20 06:00 [entrez]
PHST- 2014/06/20 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
PHST- 2014/06/19 00:00 [pmc-release]
AID - PONE-D-14-07083 [pii]
AID - 10.1371/journal.pone.0099196 [doi]
PST - epublish
SO  - PLoS One. 2014 Jun 19;9(6):e99196. doi: 10.1371/journal.pone.0099196. eCollection 
      2014.