PMID- 24946709 OWN - NLM STAT- MEDLINE DCOM- 20150526 LR - 20211021 IS - 1614-7499 (Electronic) IS - 0944-1344 (Linking) VI - 21 IP - 21 DP - 2014 Nov TI - Enhancement of in vitro high-density polyethylene (HDPE) degradation by physical, chemical, and biological treatments. PG - 12549-62 LID - 10.1007/s11356-014-3191-2 [doi] AB - Partially degraded high-density polyethylene (HDPE) was collected from plastic waste dump yard for biodegradation using fungi. Of various fungi screened, strain MF12 was found efficient in degrading HDPE by weight loss and Fourier transform infrared (FT-IR) spectrophotometric analysis. Strain MF12 was selected as efficient HDPE degraders for further studies, and their growth medium composition was optimized. Among those different media used, basal minimal medium (BMM) was suitable for the HDPE degradation by strain MF12. Strain MF12 was subjected to 28S rRNA sequence analysis and identified as Aspergillus terreus MF12. HDPE degradation was carried out using combinatorial physical and chemical treatments in conjunction to biological treatment. The high level of HDPE degradation was observed in ultraviolet (UV) and KMnO4/HCl with A. terreus MF12 treatment, i.e., FT10. The abiotic physical and chemical factors enhance the biodegradation of HDPE using A. terreus MF12. FAU - Balasubramanian, V AU - Balasubramanian V AD - Rhizosphere Biology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India, balav1982@yahoo.co.in. FAU - Natarajan, K AU - Natarajan K FAU - Rajeshkannan, V AU - Rajeshkannan V FAU - Perumal, P AU - Perumal P LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140621 PL - Germany TA - Environ Sci Pollut Res Int JT - Environmental science and pollution research international JID - 9441769 RN - 0 (Culture Media) RN - 0 (Soil Pollutants) RN - 9002-88-4 (Polyethylene) SB - IM MH - Aspergillus/*physiology MH - Biodegradation, Environmental MH - Biofilms/*growth & development MH - Culture Media MH - Microbial Viability MH - Phylogeny MH - Polyethylene/*chemistry MH - Soil Pollutants/*chemistry MH - Spectroscopy, Fourier Transform Infrared MH - Surface Properties EDAT- 2014/06/21 06:00 MHDA- 2015/05/27 06:00 CRDT- 2014/06/21 06:00 PHST- 2014/04/07 00:00 [received] PHST- 2014/06/10 00:00 [accepted] PHST- 2014/06/21 06:00 [entrez] PHST- 2014/06/21 06:00 [pubmed] PHST- 2015/05/27 06:00 [medline] AID - 10.1007/s11356-014-3191-2 [doi] PST - ppublish SO - Environ Sci Pollut Res Int. 2014 Nov;21(21):12549-62. doi: 10.1007/s11356-014-3191-2. Epub 2014 Jun 21.