PMID- 2494691
OWN - NLM
STAT- MEDLINE
DCOM- 19890427
LR  - 20190828
IS  - 0162-0886 (Print)
IS  - 0162-0886 (Linking)
VI  - 11 Suppl 1
DP  - 1989 Jan-Feb
TI  - Analysis of toxic shock syndrome isolates producing staphylococcal enterotoxins B 
      and C1 with use of southern hybridization and immunologic assays.
PG  - S75-81; discussion S81-2
AB  - A study was undertaken to evaluate the production of enterotoxin B (Ent B), 
      EntC1, and toxic shock syndrome toxin 1 (TSST-1) by isolates of Staphylococcus 
      aureus from patients with toxic shock syndrome (TSS) and from a variety of other 
      sources. Levels of toxin in culture supernatants were measured by a quantitative 
      immunodiffusion assay. Most vaginal TSS isolates produced TSST-1, either alone or 
      with EntC1. However, strains that produced EntB or EntC1 but did not express 
      TSST-1 were commonly isolated from patients with nonmenstrual TSS; EntB was 
      usually produced alone or--rarely--with EntC1. These results were confirmed by 
      probing DNA from representative isolates with an internal probe of the EntC1 gene 
      (entC1). Extensive sequence homology between entC1 and entB enabled detection of 
      both genes under conditions of high stringency. The genomic location of entC1 in 
      strains producing both EntC1 and TSST-1 varied little but was dependent on the 
      mammalian host. In contrast, the genomic location of entC1 or entB in strains 
      producing EntC1 or EntB alone was variable. These results suggest that these 
      genes are contained on mobile elements.
FAU - Bohach, G A
AU  - Bohach GA
AD  - Department of Microbiology, Medical School, University of Minnesota, Minneapolis 
      55455.
FAU - Kreiswirth, B N
AU  - Kreiswirth BN
FAU - Novick, R P
AU  - Novick RP
FAU - Schlievert, P M
AU  - Schlievert PM
LA  - eng
GR  - AI 22159/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Rev Infect Dis
JT  - Reviews of infectious diseases
JID - 7905878
RN  - 0 (Bacterial Toxins)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Superantigens)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - 39424-53-8 (enterotoxin B, staphylococcal)
RN  - 39424-54-9 (enterotoxin C, staphylococcal)
SB  - IM
MH  - Animals
MH  - *Bacterial Toxins
MH  - Base Sequence
MH  - Blotting, Southern
MH  - DNA Probes
MH  - DNA, Bacterial/genetics
MH  - Enterotoxins/*biosynthesis/genetics
MH  - Humans
MH  - Immunodiffusion
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Restriction Mapping
MH  - Sequence Homology, Nucleic Acid
MH  - Shock, Septic/*microbiology
MH  - Staphylococcus aureus/*genetics/metabolism
MH  - *Superantigens
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1093/clinids/11.supplement_1.s75 [doi]
PST - ppublish
SO  - Rev Infect Dis. 1989 Jan-Feb;11 Suppl 1:S75-81; discussion S81-2. doi: 
      10.1093/clinids/11.supplement_1.s75.