PMID- 24948597
OWN - NLM
STAT- MEDLINE
DCOM- 20150102
LR  - 20220409
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Print)
IS  - 0892-6638 (Linking)
VI  - 28
IP  - 10
DP  - 2014 Oct
TI  - Overexpression of histone deacetylases in cancer cells is controlled by interplay 
      of transcription factors and epigenetic modulators.
PG  - 4265-79
LID - 10.1096/fj.14-250654 [doi]
AB  - Histone deacetylases (HDACs) that deacetylate histone and nonhistone proteins 
      play crucial roles in a variety of cellular processes. The overexpression of 
      HDACs is reported in many cancer types and is directly linked to accelerated cell 
      proliferation and survival. However, little is known about how HDAC expression is 
      regulated in cancer cells. In this study, we found that HDAC1 and HDAC2 promoters 
      are regulated through collaborative binding of transcription factors Sp1/Sp3 and 
      epigenetic modulators, including histone H3K4 methyltransferase SET1 and histone 
      acetyltransferase p300, whose levels are also elevated in colon cancer cell lines 
      and patient samples. Interestingly, Sp1 and Sp3 differentially regulate HDAC1 and 
      HDAC2 promoter activity. In addition, Sp1/Sp3 recruits SET1 and p300 to the 
      promoters. SET1 knockdown (KD) results in a loss of the H3K4 trimethylation mark 
      at the promoters, as well as destabilizes p300 at the promoters. Conversely, p300 
      also influences SET1 recruitment and H3K4me3 level, indicating a crosstalk 
      between p300 and SET1. Further, SET1 KD reduces Sp1 binding to the HDAC1 promoter 
      through the increase of Sp1 acetylation. These results indicate that interactions 
      among transcription factors and epigenetic modulators orchestrate the activation 
      of HDAC1 and HDAC2 promoter activity in colon cancer cells.
CI  - (c) FASEB.
FAU - Yang, Hui
AU  - Yang H
AD  - Department of Anatomy and Cell Biology and.
FAU - Salz, Tal
AU  - Salz T
AD  - Department of Biochemistry and Molecular Biology, College of Medicine, University 
      of Florida, Gainesville, Florida, USA.
FAU - Zajac-Kaye, Maria
AU  - Zajac-Kaye M
AD  - Department of Anatomy and Cell Biology and.
FAU - Liao, Daiqing
AU  - Liao D
AD  - Department of Anatomy and Cell Biology and.
FAU - Huang, Suming
AU  - Huang S
AD  - Department of Biochemistry and Molecular Biology, College of Medicine, University 
      of Florida, Gainesville, Florida, USA qiuy@ufl.edu sumingh@ufl.edu.
FAU - Qiu, Yi
AU  - Qiu Y
AD  - Department of Anatomy and Cell Biology and qiuy@ufl.edu sumingh@ufl.edu.
LA  - eng
GR  - R01-HL-090589/HL/NHLBI NIH HHS/United States
GR  - R01 HL095674/HL/NHLBI NIH HHS/United States
GR  - R01-HL-095674/HL/NHLBI NIH HHS/United States
GR  - R01-HL-091929/HL/NHLBI NIH HHS/United States
GR  - R01 HL091929/HL/NHLBI NIH HHS/United States
GR  - R01 HL090589/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140619
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (Setd1A protein, human)
RN  - EC 2.3.1.48 (p300-CBP Transcription Factors)
RN  - EC 3.5.1.98 (HDAC1 protein, human)
RN  - EC 3.5.1.98 (HDAC2 protein, human)
RN  - EC 3.5.1.98 (Histone Deacetylase 1)
RN  - EC 3.5.1.98 (Histone Deacetylase 2)
SB  - IM
MH  - Colonic Neoplasms/*metabolism
MH  - *Epigenesis, Genetic
MH  - *Gene Expression Regulation, Neoplastic
MH  - HCT116 Cells
MH  - HT29 Cells
MH  - Histone Deacetylase 1/genetics/*metabolism
MH  - Histone Deacetylase 2/genetics/*metabolism
MH  - Histone-Lysine N-Methyltransferase/genetics/metabolism
MH  - Humans
MH  - Promoter Regions, Genetic
MH  - p300-CBP Transcription Factors/genetics/metabolism
PMC - PMC4202103
OTO - NOTNLM
OT  - SET1
OT  - Sp1
OT  - Sp3
OT  - p300
EDAT- 2014/06/21 06:00
MHDA- 2015/01/03 06:00
PMCR- 2015/10/01
CRDT- 2014/06/21 06:00
PHST- 2014/06/21 06:00 [entrez]
PHST- 2014/06/21 06:00 [pubmed]
PHST- 2015/01/03 06:00 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - fj.14-250654 [pii]
AID - 14-250654 [pii]
AID - 10.1096/fj.14-250654 [doi]
PST - ppublish
SO  - FASEB J. 2014 Oct;28(10):4265-79. doi: 10.1096/fj.14-250654. Epub 2014 Jun 19.