PMID- 24950754
OWN - NLM
STAT- MEDLINE
DCOM- 20150921
LR  - 20240330
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 99
IP  - 1
DP  - 2015 Jan
TI  - Investigating bacterial populations in styrene-degrading biofilters by 16S rDNA 
      tag pyrosequencing.
PG  - 3-18
LID - 10.1007/s00253-014-5868-3 [doi]
AB  - Microbial biofilms are essential components in the elimination of pollutants 
      within biofilters, yet still little is known regarding the complex relationships 
      between microbial community structure and biodegradation function within these 
      engineered ecosystems. To further explore this relationship, 16S rDNA tag 
      pyrosequencing was applied to samples taken at four time points from a 
      styrene-degrading biofilter undergoing variable operating conditions. Changes in 
      microbial structure were observed between different stages of biofilter 
      operation, and the level of styrene concentration was revealed to be a critical 
      factor affecting these changes. Bacterial genera Azoarcus and Pseudomonas were 
      among the dominant classified genera in the biofilter. Canonical correspondence 
      analysis (CCA) and correlation analysis revealed that the genera Brevundimonas, 
      Hydrogenophaga, and Achromobacter may play important roles in styrene degradation 
      under increasing styrene concentrations. No significant correlations (P > 0.05) 
      could be detected between biofilter operational/functional parameters and 
      biodiversity measurements, although biological heterogeneity within biofilms 
      and/or technical variability within pyrosequencing may have considerably affected 
      these results. Percentages of selected bacterial taxonomic groups detected by 
      fluorescence in situ hybridization (FISH) were compared to results from 
      pyrosequencing in order to assess the effectiveness and limitations of each 
      method for identifying each microbial taxon. Comparison of results revealed 
      discrepancies between the two methods in the detected percentages of numerous 
      taxonomic groups. Biases and technical limitations of both FISH and 
      pyrosequencing, such as the binding of FISH probes to non-target microbial groups 
      and lack of classification of sequences for defined taxonomic groups from 
      pyrosequencing, may partially explain some differences between the two methods.
FAU - Portune, Kevin J
AU  - Portune KJ
AD  - Research Group GI2AM, Department of Chemical Engineering, Universitat de 
      Valencia, Av. de la Universidad s/n, 46100, Burjassot, Spain, 
      kevin.portune@uv.es.
FAU - Perez, M Carmen
AU  - Perez MC
FAU - Alvarez-Hornos, F Javier
AU  - Alvarez-Hornos FJ
FAU - Gabaldon, Carmen
AU  - Gabaldon C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140621
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 44LJ2U959V (Styrene)
SB  - IM
MH  - Bacteria/*classification/*genetics/growth & development
MH  - Biofilms/*growth & development
MH  - Bioreactors/*microbiology
MH  - *Biota
MH  - Biotransformation
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Filtration/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Styrene/*metabolism
MH  - Time Factors
PMC - PMC4286631
EDAT- 2014/06/22 06:00
MHDA- 2015/09/22 06:00
PMCR- 2014/06/21
CRDT- 2014/06/22 06:00
PHST- 2014/01/31 00:00 [received]
PHST- 2014/05/31 00:00 [accepted]
PHST- 2014/05/29 00:00 [revised]
PHST- 2014/06/22 06:00 [entrez]
PHST- 2014/06/22 06:00 [pubmed]
PHST- 2015/09/22 06:00 [medline]
PHST- 2014/06/21 00:00 [pmc-release]
AID - 5868 [pii]
AID - 10.1007/s00253-014-5868-3 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2015 Jan;99(1):3-18. doi: 10.1007/s00253-014-5868-3. 
      Epub 2014 Jun 21.