PMID- 24952026
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140707
LR  - 20211021
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 66
IP  - 4
DP  - 2014 Aug
TI  - Human umbilical cord-derived mesenchymal stem cells differentiate into 
      epidermal-like cells using a novel co-culture technique.
PG  - 699-708
LID - 10.1007/s10616-013-9569-z [doi]
AB  - Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) isolated from human 
      umbilical Wharton's Jelly are a population of primitive and pluripotent cells. In 
      specific conditions, hUCMSCs can differentiate into various cells, including 
      adipocytes, osteoblasts, chondrocytes, neurocytes, and endothelial cells. 
      However, few studies have assessed their differentiation into epidermal cells in 
      vitro. To assess the potential of hUCMSCs to differentiate into epidermal cells, 
      a microporous membrane-based indirect co-culture system was developed in this 
      study. Epidermal stem cells (ESCs) were seeded on the bottom of the microporous 
      membrane, and hUCMSCs were seeded on the top of the microporous membrane. Cell 
      morphology was assessed by phase contrast microscopy, and the expression of early 
      markers of epidermal cell lineage, P63, cytokeratin19 (CK19), and beta1-integrin, 
      was determined by immunofluorescence, Western blot, and quantitative real-time 
      PCR (Q-PCR) analyses. hUCMSC morphology changed from spindle-like to oblate or 
      irregular with indirect co-culture with ESCs; they also expressed greater levels 
      P63, CK19, and beta1-integrin mRNA and protein compared to the controls (p < 0.01). 
      As compared to normal co-cultures, indirect co-culture expressed significantly 
      greater CK19 protein (p < 0.01). Thus, hUCMSCs may have the capability to 
      differentiate into the epidermal lineage in vitro, which may be accomplished 
      through this indirect co-culture model.
FAU - Li, Dongjie
AU  - Li D
AD  - Department of Burn and Plastic Surgery, the First Affiliated Hospital of PLA 
      General Hospital, Beijing, China.
FAU - Chai, Jiake
AU  - Chai J
FAU - Shen, Chuanan
AU  - Shen C
FAU - Han, Yanfu
AU  - Han Y
FAU - Sun, Tianjun
AU  - Sun T
LA  - eng
PT  - Journal Article
DEP - 20140621
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC4082775
EDAT- 2014/06/22 06:00
MHDA- 2014/06/22 06:01
PMCR- 2015/08/01
CRDT- 2014/06/22 06:00
PHST- 2012/08/15 00:00 [received]
PHST- 2013/04/16 00:00 [accepted]
PHST- 2014/06/22 06:00 [entrez]
PHST- 2014/06/22 06:00 [pubmed]
PHST- 2014/06/22 06:01 [medline]
PHST- 2015/08/01 00:00 [pmc-release]
AID - 9569 [pii]
AID - 10.1007/s10616-013-9569-z [doi]
PST - ppublish
SO  - Cytotechnology. 2014 Aug;66(4):699-708. doi: 10.1007/s10616-013-9569-z. Epub 2014 
      Jun 21.