PMID- 2495223
OWN - NLM
STAT- MEDLINE
DCOM- 19890515
LR  - 20061115
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 19
IP  - 2
DP  - 1989 Feb
TI  - Inhibition of cryoprecipitation of murine IgG3 anti-dinitrophenyl (DNP) 
      monoclonal antibodies by anionic DNP-amino acid conjugates.
PG  - 273-8
AB  - Previously we have demonstrated that eight out of nine IgG3 monoclonal antibodies 
      (mAb) obtained from autoimmune MRL-lpr/lpr mice were able to self-associate and 
      to precipitate in the cold (Gyotoku et al., J. Immunol. 1987. 138:3785). To 
      determine whether the cryoprecipitation of IgG3 mAb is enhanced or inhibited in 
      the presence of specific ligand, we have established eight IgG3 mAb reactive with 
      2,4-dinitrophenol (DNP) hapten: four mAb were obtained from fusion of spleen 
      cells of C57BL/6 mice immunized with 2,4,6-trinitrophenylated keyhole limpet 
      hemocyanin, three from 129/Sv and one from BALB/c immunized with 
      DNP-lipopolysaccharide. Five of them induced cryoglobulins composed exclusively 
      of the IgG3 mAb. The binding of negatively charged monomeric DNP-amino acid 
      conjugates completely inhibited the cryoprecipitation of all the five 
      cryoprecipitating anti-DNP IgG3 mAb, while the incubation with positively charged 
      or neutral DNP-amino acid conjugates had variable effects: increase, inhibition 
      or no change of the cryoprecipitation. In addition, positively charged DNP-amino 
      acid conjugates were able to induce the cryoprecipitation of one of the 
      non-cryoprecipitating anti-DNP IgG3 mAb. Our data showed that (a) IgG3 mAb 
      derived from non-autoimmune strains of mice, similar to IgG3 mAb derived from an 
      autoimmune MRL-lpr/lpr strain, possessed the unique property to self-associate 
      and were able to form cryoglobulins in most cases; (b) although the Fc-Fc 
      interactions of IgG3 mAb play a decisive role in IgG3 cold solubility, IgG3 
      cryoprecipitation was markedly influenced after interacting with their specific 
      ligand, depending on the charge of the hapten-amino acid conjugate. This 
      suggested that even minor interferences with the electrostatic equilibrium of the 
      IgG3 by the binding of charged hapten molecules induced dramatic changes in the 
      solubility of the IgG3 mAb at low temperature.
FAU - Spertini, F
AU  - Spertini F
AD  - Department of Pathology, World Health Organization Immunology Research and 
      Training Center, Geneva, Switzerland.
FAU - Coulie, P G
AU  - Coulie PG
FAU - Van Snick, J
AU  - Van Snick J
FAU - Davidson, E
AU  - Davidson E
FAU - Lambert, P H
AU  - Lambert PH
FAU - Izui, S
AU  - Izui S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Amino Acids)
RN  - 0 (Anions)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cryoglobulins)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Dinitrophenols)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Myeloma Proteins)
RN  - 0 (Nitrobenzenes)
RN  - 0 (Oligopeptides)
RN  - 0 (cryoprecipitate coagulum)
RN  - 63014-08-4 (DNP-peptide)
RN  - 9001-27-8 (Factor VIII)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - Amino Acids/*immunology
MH  - Animals
MH  - Anions
MH  - Antibodies, Monoclonal/*immunology/physiology
MH  - *Binding, Competitive
MH  - Cryoglobulins/biosynthesis
MH  - Dinitrobenzenes/*immunology
MH  - Dinitrophenols/immunology
MH  - Factor VIII/*antagonists & inhibitors/biosynthesis
MH  - Fibrinogen/*antagonists & inhibitors/biosynthesis
MH  - Immunoglobulin G/*immunology/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Myeloma Proteins/immunology
MH  - Nitrobenzenes/*immunology
MH  - Oligopeptides/immunology
EDAT- 1989/02/01 00:00
MHDA- 1989/02/01 00:01
CRDT- 1989/02/01 00:00
PHST- 1989/02/01 00:00 [pubmed]
PHST- 1989/02/01 00:01 [medline]
PHST- 1989/02/01 00:00 [entrez]
AID - 10.1002/eji.1830190209 [doi]
PST - ppublish
SO  - Eur J Immunol. 1989 Feb;19(2):273-8. doi: 10.1002/eji.1830190209.