PMID- 24954331
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20221207
IS  - 1976-2437 (Electronic)
IS  - 0513-5796 (Print)
IS  - 0513-5796 (Linking)
VI  - 55
IP  - 4
DP  - 2014 Jul
TI  - Resolution of ambiguous HLA genotyping in korean by multi-group-specific 
      sequence-based typing.
PG  - 1005-13
LID - 10.3349/ymj.2014.55.4.1005 [doi]
AB  - PURPOSE: To evaluate a multi-group-specific sequence-based typing (SBT) method 
      for resolving ambiguous results from human leukocyte antigen (HLA) genotyping. 
      MATERIALS AND METHODS: A total of 50 samples that showed ambiguous genotypes for 
      at least two HLA loci from HLA-A, -B, -C and -DRB1 by the conventional SBT assay 
      were evaluated using a new SBT test, the AVITA plus assay. The most likely HLA 
      genotypes for the respective samples considering allele frequencies in Korean 
      were concordant between the AVITA and conventional SBT assays. RESULTS: An 
      average of 3.3 loci among the HLA-A, -B, -C and -DRB1 loci per sample gave 
      results with two or more possible allele combinations with the conventional SBT, 
      and 48 (96.0%) out of 50 showed reduced numbers of possible genotypes for at 
      least one HLA locus with the AVITA. A total of 41, 43, 42, and 38 cases among the 
      50 samples showed ambiguous results for HLA-A, -B, -C, and -DRB1 typing by the 
      conventional SBT, respectively. The average numbers of possible allele 
      combinations for the respective four HLA loci were 8.2, 6.7, 5.9, and 3.2, and 
      they were reduced to 1.5, 2.2, 4.4, and 1.8, respectively, by the AVITA. 
      Ambiguity was resolved by the AVITA in 33 (80.5%), 31 (72.1%), 17 (40.5%) and 28 
      (73.7%) samples among the ambiguous cases from the conventional SBT for HLA-A, 
      -B, -C, and -DRB1 typing, respectively. CONCLUSION: The multi-group-specific SBT 
      method considerably reduced the number of ambiguous results, and thus may be 
      useful for accurate HLA typing in clinical laboratories.
FAU - Park, Yongjung
AU  - Park Y
AD  - Department of Laboratory Medicine, Severance Hospital, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Yoon, Cha Eun
AU  - Yoon CE
AD  - Biowithus Life Science Institute, Seoul, Korea.
FAU - Kwon, Oh-Joong
AU  - Kwon OJ
AD  - College of Animal Bioscience & Technology, Konkuk University, Seoul, Korea.
FAU - Kim, Yu-Seun
AU  - Kim YS
AD  - Division of Transplantation Surgery, Department of Surgery,The Research Institute 
      for Transplantation, Severance Hospital, Yonsei University College of Medicine, 
      Seoul, Korea.
FAU - Kim, Hyon-Suk
AU  - Kim HS
AD  - Department of Laboratory Medicine, Severance Hospital, Yonsei University College 
      of Medicine, Seoul, Korea. kimhs54@yuhs.ac.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Yonsei Med J
JT  - Yonsei medical journal
JID - 0414003
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Asian People/genetics
MH  - Base Sequence
MH  - Gene Frequency/genetics
MH  - Genotype
MH  - HLA Antigens/*genetics
MH  - Histocompatibility Testing
MH  - Humans
MH  - Polymerase Chain Reaction
PMC - PMC4075361
OTO - NOTNLM
OT  - Human leukocyte antigen
OT  - ambiguity
OT  - high resolution
OT  - multi-group-specific PCR
OT  - sequence-based typing
COIS- The authors have no financial conflicts of interest.
EDAT- 2014/06/24 06:00
MHDA- 2015/04/10 06:00
PMCR- 2014/07/01
CRDT- 2014/06/24 06:00
PHST- 2014/06/24 06:00 [entrez]
PHST- 2014/06/24 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - 2014071005 [pii]
AID - 10.3349/ymj.2014.55.4.1005 [doi]
PST - ppublish
SO  - Yonsei Med J. 2014 Jul;55(4):1005-13. doi: 10.3349/ymj.2014.55.4.1005.