PMID- 24955635
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140624
LR  - 20211021
IS  - 2079-4983 (Print)
IS  - 2079-4983 (Electronic)
IS  - 2079-4983 (Linking)
VI  - 3
IP  - 3
DP  - 2012 Sep 5
TI  - Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped 
      with Silica Particles and Fluorescent Compounds.
PG  - 601-14
LID - 10.3390/jfb3030601 [doi]
AB  - Biopolymers are easily denatured by heating, a change in pH or chemical 
      substances when they are immobilized on a substrate. To prevent denaturation of 
      biopolymers, we developed a method to trap a polynucleotide on a substrate by 
      hydrogen bonding using silica particles with surfaces modified by aminoalkyl 
      chains ([A-AM silane]/SiO2). [A-AM silane]/SiO2 was synthesized by silane 
      coupling reaction of N-2-(aminoethyl)-3-aminopropyltrimethoxysilane (A-AM silane) 
      with SiO2 particles with a diameter of 5 mum at 100  degrees C for 20 min. The surface 
      chemical structure of [A-AM silane]/SiO2 was characterized by Fourier transform 
      infrared spectroscopy and molecular orbital calculations. The surface of the 
      silica particles was modified with A-AM silane and primary amine groups were 
      formed. [A-AM silane]/SiO2 was trapped with single-stranded nucleic acids 
      [(Poly-X; X = A (adenine), G (guanine) and C (cytosine)] in PBS solution at 37  degrees C 
      for 1 h. The single-stranded nucleic acids were trapped on the surface of the 
      [A-AM silane]/SiO2 by hydrogen bonding to form conjugated materials. The 
      resulting complexes were further conjugated by derivatives of acridine orange 
      (AO) as fluorescent labels under the same conditions to form [AO:Poly-X:A-AM 
      silane]/SiO2 complexes. Changes in the fluorescence intensity of these complexes 
      originating from interactions between the single-stranded nucleic acid and 
      aromatic compounds were also evaluated. The change in intensity displayed the 
      order [AO: Poly-G: A-AM silane]/SiO2 > [AO:Poly-A:A-AM silane]/SiO2 >> 
      [AO:Poly-C:A-AM silane]/SiO2. This suggests that the single-stranded nucleic 
      acids conjugated with aminoalkyl chains on the surfaces of SiO2 particles and the 
      change in fluorescence intensity reflected the molecular interaction between AO 
      and the nucleic-acid base in a polynucleotide.
FAU - Isoda, T
AU  - Isoda T
AD  - Department of Life and Environment Engineering, Faculty of Environmental 
      Engineering, University of Kitakyushu, 1-1, Hibikino, Wakamatsu, Kitakyushu 
      808-0135, Japan. isoda@env.kitakyu-u.ac.jp.
FAU - Maeda, R
AU  - Maeda R
AD  - Department of Life and Environment Engineering, Graduate School of Environmental 
      Engineering, University of Kitakyushu, 1-1, Hibikino, Wakamatsu, Kitakyushu 
      808-0135, Japan. s1mab008@eng.kitakyu-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20120905
PL  - Switzerland
TA  - J Funct Biomater
JT  - Journal of functional biomaterials
JID - 101570734
PMC - PMC4031003
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2012/09/01
CRDT- 2014/06/24 06:00
PHST- 2012/07/13 00:00 [received]
PHST- 2012/08/07 00:00 [revised]
PHST- 2012/08/21 00:00 [accepted]
PHST- 2014/06/24 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - jfb3030601 [pii]
AID - jfb-03-00601 [pii]
AID - 10.3390/jfb3030601 [doi]
PST - epublish
SO  - J Funct Biomater. 2012 Sep 5;3(3):601-14. doi: 10.3390/jfb3030601.