PMID- 24955930
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140624
LR  - 20211021
IS  - 2079-4983 (Print)
IS  - 2079-4983 (Electronic)
IS  - 2079-4983 (Linking)
VI  - 1
IP  - 1
DP  - 2010 Oct 22
TI  - Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated 
      Chitooligosaccharides on the Osteosarcoma MG63 Cell Line.
PG  - 3-13
LID - 10.3390/jfb1010003 [doi]
AB  - Phosphorylated chitooligosaccharides (P-COS) were prepared using a H3PO4, P2O5, 
      Et3PO4 and hexanol solvent system. The P-COS were characterized by Fourier 
      Transform Infrared Spectroscopy (FT-IR), Thermo gravimetric-Differential Thermal 
      Analyzer (TG-DTA), 13C NMR, 31P NMR, X-ray diffraction analysis, solubility 
      studies, biocompatibility and Alkaline Phosphatase Activity (ALP). The results 
      reveal that phosphorylation occurred at the C3 and C6 position of OH groups and 
      the C2 position of NH2 group. FT-IR confirmed no decomposition in pyranose ring 
      in P-COS even with heating and treatment in acidic conditions. The amorphous 
      nature of P-COS was confirmed by X-ray diffraction analysis. Further, the 
      biocompatibility and alkaline phosphatase activity of P-COS were checked against 
      the osteosarcoma MG63 cell line at different concentrations and no cytotoxicity 
      was observed. After 12 h and 24 h of incubation, the ALP activity of P-COS was 
      higher compared with the control group. These results suggest that P-COS is a 
      biocompatible material and in future P-COS could open up a number of promising 
      pharmaceutical and clinical applications to mankind.
FAU - Venkatesan, Jayachandran
AU  - Venkatesan J
AD  - Department of Chemistry, Pukyong National University, Busan 608-737, Korea. 
      venkatjchem@gmail.com.
FAU - Pangestuti, Ratih
AU  - Pangestuti R
AD  - Department of Chemistry, Pukyong National University, Busan 608-737, Korea. 
      pangesturth@yahoo.com.
FAU - Qian, Zhong-Ji
AU  - Qian ZJ
AD  - Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, 
      Korea. qianzhongji@hanmail.net.
FAU - Ryu, BoMi
AU  - Ryu B
AD  - Department of Chemistry, Pukyong National University, Busan 608-737, Korea. 
      ryu.bomi@gmail.com.
FAU - Kim, Se-Kwon
AU  - Kim SK
AD  - Department of Chemistry, Pukyong National University, Busan 608-737, Korea. 
      sknkim@pknu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20101022
PL  - Switzerland
TA  - J Funct Biomater
JT  - Journal of functional biomaterials
JID - 101570734
PMC - PMC4030895
EDAT- 2010/01/01 00:00
MHDA- 2010/01/01 00:01
PMCR- 2010/12/01
CRDT- 2014/06/24 06:00
PHST- 2010/08/26 00:00 [received]
PHST- 2010/10/07 00:00 [revised]
PHST- 2010/10/20 00:00 [accepted]
PHST- 2014/06/24 06:00 [entrez]
PHST- 2010/01/01 00:00 [pubmed]
PHST- 2010/01/01 00:01 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - jfb1010003 [pii]
AID - jfb-01-00003 [pii]
AID - 10.3390/jfb1010003 [doi]
PST - epublish
SO  - J Funct Biomater. 2010 Oct 22;1(1):3-13. doi: 10.3390/jfb1010003.