PMID- 2495723
OWN - NLM
STAT- MEDLINE
DCOM- 19890524
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 134
IP  - 4
DP  - 1989 Apr
TI  - Light chain deposition disease derived from the kappa I light chain subgroup. 
      Biochemical characterization.
PG  - 749-54
AB  - The authors biochemically analyzed the nonamyloidotic light chain deposits, the 
      first studied in this way, from a patient with systemic kappa light chain 
      deposition disease (LCDD). The light chain deposits from myocardium were 
      extracted in 6 M guanidine-HCl under reducing conditions, partially purified by 
      column chromatography, and analyzed by immunoblotting and amino-terminal 
      sequencing. The extracted material contained four main bands reactive with 
      anti-kappa antibody: intact kappa light chain (MW, 28 kd), under reducing 
      conditions, and 3 fragments (MW, 20, 16, and 15 kd). As revealed by the 
      aminoterminal sequencing performed on three of the four bands, the intact light 
      chain molecule and two fragments belong to the kappa I subgroup. Thus, similar to 
      light chain amyloid (AL), the deposits in LCDD are derived from both intact light 
      chain and fragments. Unlike in AL, amyloid P component was not detected in the 
      deposits of this patient or those examined previously. The differences 
      demonstrated thus far between AL and LCDD are the lack of fibrils and amyloid P 
      component in LCDD, suggesting that local tissue factors may be responsible for 
      different processing of the light chain deposits in LCDD.
FAU - Picken, M M
AU  - Picken MM
AD  - Department of Pathology, New York University Medical Center, New York 10016.
FAU - Frangione, B
AU  - Frangione B
FAU - Barlogie, B
AU  - Barlogie B
FAU - Luna, M
AU  - Luna M
FAU - Gallo, G
AU  - Gallo G
LA  - eng
GR  - AM 01431/AM/NIADDK NIH HHS/United States
GR  - AM 02594/AM/NIADDK NIH HHS/United States
GR  - CA 37161/CA/NCI NIH HHS/United States
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (Immunoglobulin kappa-Chains)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Hypergammaglobulinemia/*metabolism
MH  - Immunoblotting
MH  - Immunoglobulin Light Chains/*metabolism
MH  - Immunoglobulin kappa-Chains/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Molecular Sequence Data
MH  - Myocardium/*metabolism
PMC - PMC1879798
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
PMCR- 1989/10/01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
PHST- 1989/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1989 Apr;134(4):749-54.