PMID- 24959122
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140624
LR  - 20211021
IS  - 1662-5129 (Print)
IS  - 1662-5129 (Electronic)
IS  - 1662-5129 (Linking)
VI  - 8
DP  - 2014
TI  - Biodistribution of adeno-associated virus serotype 9 (AAV9) vector after 
      intrathecal and intravenous delivery in mouse.
PG  - 42
LID - 10.3389/fnana.2014.00042 [doi]
LID - 42
AB  - Adeno-associated virus serotype 9 (AAV9)-mediated gene transfer has been reported 
      in central nervous system (CNS) and peripheral tissues. The current study 
      compared the pattern of expression of Green Fluorescent Protein (GFP) across the 
      mouse CNS and selected peripheral tissues after intrathecal (i.t.) or intravenous 
      (i.v.) delivery of equivalent doses of single-stranded AAV9 vector. After i.t. 
      delivery, GFP immunoreactivity (-ir) was observed in spinal neurons, primary 
      afferent fibers and corresponding primary sensory neurons at all spinal levels. 
      Robust transduction was seen in small and large dorsal root ganglion (DRG) 
      neurons as well as trigeminal and vagal primary afferent neurons. Transduction 
      efficiency in sensory ganglia was substantially lower in i.v. treated mice. In 
      brain, i.v. delivery yielded GFP-immunoreactivity (-ir) primarily in spinal 
      trigeminal tract, pituitary, and scattered isolated neurons and astrocytes. In 
      contrast, after i.t. delivery, GFP-ir was widespread throughout CNS, with greater 
      intensity and more abundant neuropil-like staining at 6 weeks compared to 3 
      weeks. Brain regions with prominent GFP-ir included cranial nerve nuclei, ventral 
      pons, cerebellar cortex, hippocampus, pituitary, choroid plexus, and selected 
      nuclei of midbrain, thalamus and hypothalamus. In cortex, GFP-ir was associated 
      with blood vessels, and was seen in both neurons and astrocytes. In the 
      periphery, GFP-ir in colon and ileum was present in the enteric nervous system in 
      both i.v. and i.t. treated mice. Liver and adrenal cortex, but not adrenal 
      medulla, also showed abundant GFP-ir after both routes of delivery. In summary, 
      i.t. delivery yielded higher transduction efficiency in sensory neurons and the 
      CNS. The observation of comparable gene transfer to peripheral tissues using the 
      two routes indicates that a component of i.t. delivered vector is redistributed 
      from the subarachnoid space to the systemic circulation.
FAU - Schuster, Daniel J
AU  - Schuster DJ
AD  - Departments of Neuroscience, University of Minnesota Minneapolis, MN, USA.
FAU - Dykstra, Jaclyn A
AU  - Dykstra JA
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota Saint 
      Paul, MN, USA.
FAU - Riedl, Maureen S
AU  - Riedl MS
AD  - Departments of Neuroscience, University of Minnesota Minneapolis, MN, USA.
FAU - Kitto, Kelley F
AU  - Kitto KF
AD  - Departments of Neuroscience, University of Minnesota Minneapolis, MN, USA.
FAU - Belur, Lalitha R
AU  - Belur LR
AD  - Departments of Genetics Cell Biology and Development, University of Minnesota 
      Minneapolis, MN, USA.
FAU - McIvor, R Scott
AU  - McIvor RS
AD  - Departments of Genetics Cell Biology and Development, University of Minnesota 
      Minneapolis, MN, USA.
FAU - Elde, Robert P
AU  - Elde RP
AD  - Departments of Neuroscience, University of Minnesota Minneapolis, MN, USA.
FAU - Fairbanks, Carolyn A
AU  - Fairbanks CA
AD  - Departments of Neuroscience, University of Minnesota Minneapolis, MN, USA ; 
      Departments of Pharmaceutics, University of Minnesota Minneapolis, MN, USA.
FAU - Vulchanova, Lucy
AU  - Vulchanova L
AD  - Departments of Neuroscience, University of Minnesota Minneapolis, MN, USA.
LA  - eng
GR  - R21 DA025164/DA/NIDA NIH HHS/United States
GR  - F31 NS063634/NS/NINDS NIH HHS/United States
GR  - R01 DE021996/DE/NIDCR NIH HHS/United States
GR  - T32 GM008471/GM/NIGMS NIH HHS/United States
GR  - P01 HD032652/HD/NICHD NIH HHS/United States
PT  - Journal Article
DEP - 20140610
PL  - Switzerland
TA  - Front Neuroanat
JT  - Frontiers in neuroanatomy
JID - 101477943
PMC - PMC4051274
OTO - NOTNLM
OT  - AAV9
OT  - CNS
OT  - DRG
OT  - adeno-associated
OT  - intrathecal
OT  - intravenous
OT  - trigeminal
EDAT- 2014/06/25 06:00
MHDA- 2014/06/25 06:01
PMCR- 2014/01/01
CRDT- 2014/06/25 06:00
PHST- 2014/03/10 00:00 [received]
PHST- 2014/05/16 00:00 [accepted]
PHST- 2014/06/25 06:00 [entrez]
PHST- 2014/06/25 06:00 [pubmed]
PHST- 2014/06/25 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.3389/fnana.2014.00042 [doi]
PST - epublish
SO  - Front Neuroanat. 2014 Jun 10;8:42. doi: 10.3389/fnana.2014.00042. eCollection 
      2014.