PMID- 24959274
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 8
IP  - 1
DP  - 2014 Jul
TI  - Voltage-gated sodium channels were differentially expressed in human normal 
      prostate, benign prostatic hyperplasia and prostate cancer cells.
PG  - 345-350
AB  - Voltage-gated sodium channels (VGSCs) are expressed not only in excitable cells 
      but also in numerous metastatic cells, particularly in certain types of cancer 
      cells. In some types of cancer, including prostate cancer, the expression of 
      VGSCs is associated with cancer migration, invasion and metastasis in vivo. 
      However, the detailed expression profiles of VGSC alpha subunits in normal human 
      prostate, in prostatic hyperplasia and prostatic cancer remain controversial. In 
      the present study, quantitative polymerase chain reaction was used to 
      systematically detect all subtypes of VGSC alpha subunits in normal human prostate, 
      benign prostatic hyperplasia (BPH) and prostate cancer cells. The expression 
      profile of VGSC alpha subunits was observed to differ between these cell types. 
      Nav1.5 was the major isoform expressed in normal human prostate tissue, while 
      Nav1.5 and Nav1.2 were the predominant isoforms in BPH tissue. However, in PC-3 
      and LNCaP cells, two typical prostate cancer cell lines, Nav1.6 and Nav1.7 were 
      abundantly expressed. By comparing the relative expression levels of Nav1.5, 
      Nav1.6 and Nav1.7 in these cells, the mRNA levels of Nav1.6 and Nav1.7 were 
      identified to be 6- to 27-fold higher in PC-3 and LNCaP cells than in either 
      normal or BPH samples (P<0.05); however, Nav1.5 mRNA levels were relatively lower 
      compared with those of Nav1.6 or Nav1.7 in all cells analyzed. To confirm whether 
      Nav1.6 and Nav1.7 expression in cancer cells was functional, a patch-clamp 
      technique was used to record whole-cell currents. A tetrodotoxin-sensitive sodium 
      current was successfully recorded in PC-3 cells, but not in LNCaP cells. It was 
      concluded that although all types of VGSC alpha subunits exhibited low expression 
      levels in normal prostate and BPH cells, both Nav1.6 and Nav1.7 were 
      significantly upregulated in the prostate cancer cell lines, suggesting these 
      subtypes may be potential diagnostic markers and therapeutic targets for certain 
      types of prostate cancer in humans.
FAU - Shan, Bin
AU  - Shan B
AD  - Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, 
      P.R. China.
FAU - Dong, Mei
AU  - Dong M
AD  - Department of Surgery, The Affiliated Hospital of Hebei Science and Technology 
      University, Shijiazhuang, Hebei 050018, P.R. China.
FAU - Tang, He
AU  - Tang H
AD  - Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, 
      P.R. China.
FAU - Wang, Na
AU  - Wang N
AD  - Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, 
      P.R. China.
FAU - Zhang, Jin
AU  - Zhang J
AD  - Department of Urology, The First Hospital of Shijiazhuang, Shijiazhuang, Hebei 
      050011, P.R. China.
FAU - Yan, Changqing
AU  - Yan C
AD  - Department of Hepatobiliary Surgery, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050017, P.R. China.
FAU - Jiao, Xiaocui
AU  - Jiao X
AD  - Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, 
      P.R. China.
FAU - Zhang, Hailin
AU  - Zhang H
AD  - Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, 
      P.R. China.
FAU - Wang, Chuan
AU  - Wang C
AD  - Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, 
      P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20140502
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC4063587
OTO - NOTNLM
OT  - benign prostatic hyperplasia
OT  - cancer
OT  - mRNA
OT  - prostate
OT  - voltage-gated sodium channel
EDAT- 2014/06/25 06:00
MHDA- 2014/06/25 06:01
PMCR- 2014/05/02
CRDT- 2014/06/25 06:00
PHST- 2013/10/30 00:00 [received]
PHST- 2014/04/10 00:00 [accepted]
PHST- 2014/06/25 06:00 [entrez]
PHST- 2014/06/25 06:00 [pubmed]
PHST- 2014/06/25 06:01 [medline]
PHST- 2014/05/02 00:00 [pmc-release]
AID - ol-08-01-0345 [pii]
AID - 10.3892/ol.2014.2110 [doi]
PST - ppublish
SO  - Oncol Lett. 2014 Jul;8(1):345-350. doi: 10.3892/ol.2014.2110. Epub 2014 May 2.