PMID- 24961542
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20140714
IS  - 1873-6823 (Electronic)
IS  - 0741-8329 (Linking)
VI  - 48
IP  - 5
DP  - 2014 Aug
TI  - Chronic ethanol exposure increases the non-dominant glucocorticoid, 
      corticosterone, in the near-term pregnant guinea pig.
PG  - 477-81
LID - S0741-8329(14)00105-0 [pii]
LID - 10.1016/j.alcohol.2014.06.005 [doi]
AB  - Maternal-fetal signaling is critical for optimal fetal development and postnatal 
      outcomes. Chronic ethanol exposure alters programming of the fetal 
      hypothalamic-pituitary-adrenal (HPA) axis, resulting in a myriad of neurochemical 
      and behavioral alterations in postnatal life. Based on a recent study which 
      showed that human intra-partum fetal stress increased fetal secretion of 
      corticosterone, the non-dominant glucocorticoid, this investigation tested the 
      hypothesis that an established model of HPA axis programming, chronic maternal 
      ethanol administration to the pregnant guinea pig, would result in preferential 
      elevation of corticosterone, which is also the non-dominant glucocorticoid. 
      Starting on gestational day (GD) 2, guinea pigs received oral administration of 
      ethanol (4 g/kg maternal body weight/day) or isocaloric-sucrose/pair-feeding. 
      Each treatment was administered daily and continued until GD 45, 55, or 65 
      (approximately 3 days pre-term), when pregnant animals were euthanized and 
      fetuses delivered by Caesarean section. Maternal and fetal plasma samples were 
      collected. After sample preparation (protein precipitation and C-18 solid phase 
      extraction), plasma cortisol and corticosterone concentrations were determined 
      simultaneously by liquid chromatography coupled to tandem mass spectrometry. As 
      predicted, chronic ethanol exposure increased both fetal and maternal plasma 
      corticosterone concentration in late gestation. In contrast, plasma cortisol did 
      not differ across maternal treatments in maternal or fetal samples. The plasma 
      concentration of both maternal glucocorticoids increased with gestational age. 
      Thus, corticosterone, the non-dominant glucocorticoid, but not cortisol, was 
      elevated by chronic ethanol exposure, which may have effects on HPA function in 
      later life.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Hewitt, Amy J
AU  - Hewitt AJ
AD  - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, 
      ON, Canada K7L 3N6.
FAU - Dobson, Christine C
AU  - Dobson CC
AD  - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, 
      ON, Canada K7L 3N6.
FAU - Brien, James F
AU  - Brien JF
AD  - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, 
      ON, Canada K7L 3N6; Centre for Neuroscience Studies, Queen's University, 
      Kingston, ON, Canada K7L 3N6.
FAU - Wynne-Edwards, Katherine E
AU  - Wynne-Edwards KE
AD  - Department of Comparative Biology and Experimental Medicine, Faculty of 
      Veterinary Medicine, and Hotchkiss Brain Institute, University of Calgary, 
      Calgary, AB, Canada T2N 4N1.
FAU - Reynolds, James N
AU  - Reynolds JN
AD  - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, 
      ON, Canada K7L 3N6; Centre for Neuroscience Studies, Queen's University, 
      Kingston, ON, Canada K7L 3N6. Electronic address: jnr@queensu.ca.
LA  - eng
GR  - ELA 80227/Canadian Institutes of Health Research/Canada
GR  - MOP 81185/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140607
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 3K9958V90M (Ethanol)
RN  - W980KJ009P (Corticosterone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Corticosterone/*blood
MH  - Ethanol/*administration & dosage
MH  - Female
MH  - Fetal Blood/chemistry
MH  - Fetal Development/drug effects
MH  - Guinea Pigs
MH  - Hydrocortisone/blood
MH  - Hypothalamo-Hypophyseal System/drug effects
MH  - Pituitary-Adrenal System/drug effects
MH  - Pregnancy
MH  - Pregnancy, Animal/*drug effects
OTO - NOTNLM
OT  - Chronic ethanol exposure
OT  - Corticosterone
OT  - Cortisol
OT  - Maternal-fetal stress
EDAT- 2014/06/26 06:00
MHDA- 2015/02/24 06:00
CRDT- 2014/06/26 06:00
PHST- 2014/06/26 06:00 [entrez]
PHST- 2014/06/26 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
AID - S0741-8329(14)00105-0 [pii]
AID - 10.1016/j.alcohol.2014.06.005 [doi]
PST - ppublish
SO  - Alcohol. 2014 Aug;48(5):477-81. doi: 10.1016/j.alcohol.2014.06.005. Epub 2014 Jun 
      7.