PMID- 24961772
OWN - NLM
STAT- MEDLINE
DCOM- 20151008
LR  - 20141219
IS  - 1740-0929 (Electronic)
IS  - 1344-3941 (Linking)
VI  - 85
IP  - 12
DP  - 2014 Dec
TI  - Low expression of the antibacterial factor L-amino acid oxidase in bovine mammary 
      gland.
PG  - 976-80
LID - 10.1111/asj.12237 [doi]
AB  - In the mouse, L-amino acid oxidase (LAO) produces hydrogen peroxide by utilizing 
      free amino acids and is a proven antibacterial factor in mammary glands. 
      Mastitis, a bacterial infection of the mammary gland, is the most frequent 
      disease in dairy cattle. Here, we investigate whether LAO is expressed in the 
      mammary gland of dairy cattle and is antibacterial. In dairy cattle, the 
      expression level of LAO mRNA in the mammary gland was considerably lower than 
      that in mice, and LAO activity was not observed in cattle milk that produced 
      hydrogen peroxide. The expression of LAO mRNA was also low in Japanese Black 
      cattle, the same as in Holstein cattle. A higher LAO mRNA expression was observed 
      in the mastitis glands than in the lactating glands. Furthermore, spleen and 
      lymph nodes expressed high levels of LAO mRNA in dairy cattle. We conclude that 
      mammary glands in dairy cattle have lower ability to express the LAO gene 
      compared to that in mice, which may result in a high incidence of mastitis.
CI  - (c) 2014 Japanese Society of Animal Science.
FAU - Nagaoka, Kentaro
AU  - Nagaoka K
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Fuchu, Japan.
FAU - Zhang, Haolin
AU  - Zhang H
FAU - Arakuni, Masahiro
AU  - Arakuni M
FAU - Taya, Kazuyoshi
AU  - Taya K
FAU - Watanabe, Gen
AU  - Watanabe G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140624
PL  - Australia
TA  - Anim Sci J
JT  - Animal science journal = Nihon chikusan Gakkaiho
JID - 100956805
RN  - 0 (Amino Acids)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (RNA, Messenger)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Cattle
MH  - Female
MH  - *Gene Expression
MH  - Hydrogen Peroxide/metabolism
MH  - L-Amino Acid Oxidase/*genetics/physiology
MH  - Lactation/genetics/metabolism
MH  - Mammary Glands, Animal/*enzymology
MH  - Mastitis, Bovine/*enzymology/*genetics
MH  - Mice
MH  - Mice, Inbred ICR
MH  - RNA, Messenger/metabolism
OTO - NOTNLM
OT  - amino acid
OT  - hydrogen peroxide
OT  - innate immune system
OT  - mastitis
EDAT- 2014/06/26 06:00
MHDA- 2015/10/09 06:00
CRDT- 2014/06/26 06:00
PHST- 2013/11/26 00:00 [received]
PHST- 2014/03/03 00:00 [accepted]
PHST- 2014/06/26 06:00 [entrez]
PHST- 2014/06/26 06:00 [pubmed]
PHST- 2015/10/09 06:00 [medline]
AID - 10.1111/asj.12237 [doi]
PST - ppublish
SO  - Anim Sci J. 2014 Dec;85(12):976-80. doi: 10.1111/asj.12237. Epub 2014 Jun 24.