PMID- 24962969
OWN - NLM
STAT- MEDLINE
DCOM- 20160119
LR  - 20200930
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 103
IP  - 3
DP  - 2015 Apr
TI  - Peroxisome proliferator activated receptor gamma loaded dental implant improves 
      osteogenesis of rat mandible.
PG  - 587-95
LID - 10.1002/jbm.b.33207 [doi]
AB  - Peroxisome proliferator activated receptor gamma (PPARgamma) has been known for their 
      anti-inflammatory effects. But the application of this molecule in 
      implant-induced inflammation has not been clearly studied yet. Here, we 
      determined in vivo anti-inflammatory and osteogenic effects of PPARgamma coated 
      dental implant in the rat mandible. We used chitosan gold nanoparticles (Ch-GNPs) 
      as a non viral vector to carry PPARgamma plasmid DNA. Ch-GNPs were conjugated with 
      PPARgamma plasmid DNA through a coacervation process. Conjugation was cast over 
      titanium (Ti) implants (4.5 x 0.8 mm) by dipping, and implants were installed in 
      rat mandibles. One, 2, 3, and 6 weeks post-implantation, mandibles were examined 
      by microcomputed tomography (microCT), immunohistochemistry, hematoxylin & eosin, and 
      tartrate resistance acid phosphatase (TRAP) staining. In vivo Ch-GNPs/PPARgammacoated 
      implants were associated with inhibition of implant induced inflammatory 
      molecules interleukin-1beta and receptor activator of nuclear factor kappa-B ligand 
      and enhanced expression of osteogenic molecules like bone morphogenetic protein 2 
      and 7 (BMP-2/-7) by up-regulating anti-oxidant molecules heme oxygenase-1. microCT 
      demonstrated that PPARgamma overexpression increased the density and volume of newly 
      formed bone surrounding the implants compared to control (n = 4; p < 0.05). Also, 
      PPARgamma reduced the number of TRAP positive cells. These results support the view 
      that PPARgamma overexpression diminishes inflammation and enhances osteogenesis 
      around the dental implants. Thus, implant coated with anti-inflammatory molecules 
      could have a significant utilization for the preparation of new biomaterials and 
      may serve as prosthetic materials in patients suffering from inflammatory bone 
      disease.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Bhattarai, Govinda
AU  - Bhattarai G
AD  - Department of Oral Biochemistry, Institute of Oral Bioscience, School of 
      Dentistry, Chonbuk National University, Jeonju, Korea.
FAU - Lee, Young-Hee
AU  - Lee YH
FAU - Yi, Ho-Keun
AU  - Yi HK
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140625
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (DNA, Recombinant)
RN  - 0 (Dental Implants)
RN  - 0 (Dental Materials)
RN  - 0 (Drug Carriers)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NF-kappa B)
RN  - 0 (PPAR gamma)
RN  - 7440-57-5 (Gold)
RN  - 9012-76-4 (Chitosan)
RN  - D1JT611TNE (Titanium)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Hmox1 protein, rat)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Proteins/biosynthesis
MH  - Bone Screws
MH  - Chitosan
MH  - Coated Materials, Biocompatible/*therapeutic use
MH  - DNA, Recombinant/administration & dosage
MH  - Dental Implantation, Endosseous/*instrumentation
MH  - *Dental Implants
MH  - Dental Materials/*therapeutic use
MH  - Drug Carriers
MH  - Foreign-Body Reaction/metabolism/pathology/prevention & control
MH  - Gold
MH  - Heme Oxygenase (Decyclizing)/biosynthesis
MH  - Inflammation/prevention & control
MH  - Interleukin-1beta/analysis
MH  - Materials Testing
MH  - Metal Nanoparticles
MH  - NF-kappa B/analysis
MH  - Osseointegration/physiology
MH  - Osteoclasts/metabolism
MH  - Osteogenesis, Distraction/*instrumentation
MH  - PPAR gamma/administration & dosage/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Titanium
OTO - NOTNLM
OT  - PPARgamma
OT  - dental implant
OT  - gene delivered implant
OT  - inflammation
OT  - osteogenesis
EDAT- 2014/06/26 06:00
MHDA- 2016/01/20 06:00
CRDT- 2014/06/26 06:00
PHST- 2013/12/10 00:00 [received]
PHST- 2014/04/10 00:00 [revised]
PHST- 2014/05/08 00:00 [accepted]
PHST- 2014/06/26 06:00 [entrez]
PHST- 2014/06/26 06:00 [pubmed]
PHST- 2016/01/20 06:00 [medline]
AID - 10.1002/jbm.b.33207 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2015 Apr;103(3):587-95. doi: 
      10.1002/jbm.b.33207. Epub 2014 Jun 25.