PMID- 24966959
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20221207
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 7
IP  - 5
DP  - 2014
TI  - Gene amplification of EGFR and its clinical significance in various cervical 
      (lesions) lesions using cytology and FISH.
PG  - 2477-83
AB  - The purpose of this study was to investigate the gene amplification and clinical 
      significance of the epithelial growth factor receptor (EGFR) gene in cervical 
      lesions. This study was designed to detect the EGFR gene amplification by 
      liquid-based cytology and fluorescence in situ hybridization (FISH) techniques in 
      78 cases of cervical various lesions [28 cases of normal control cervix, 26 low 
      grade squamous intraepithelial lesion (LSIL) and 25 high grade squamous 
      intraepithelial lesion (HSIL)]. Positive gene amplification rates of the EGFR 
      gene in normal cervix, LSIL and HSIL were 7.14%, 23.08% and 62.50%, respectively. 
      There was a significantly difference between HSIL and normal control cervix or 
      LSIL (P<0.01). At the same time, the gene amplification rate of EGFR was also 
      significantly different between the cases of LSIL with positive follow-up and 
      negative follow-up (P<0.01). In addition, the correlation between EGFR gene 
      amplification and HPV viral load in the same sample was evaluated in some cases. 
      Then the increase of EGFR gene amplification in HSILs and LSILs with positive 
      follow-up suggests that EGFR gene amplification is associated with the severity 
      of cytologic findings. Therefore, detection of EGFR gene may provide an objective 
      genetic test for the assessment of cells in Pap smears and serves as a screening 
      marker for HSILs or LSILs, which may help determine the progressive potential of 
      individual lesions.
FAU - Li, Qing
AU  - Li Q
AD  - Department of Pathology, Nanjing Jinling Hospital NJ 210002, China ; Department 
      of Pathology, Nanjing Maternity and Child Health Hospital, Nanjing Medical 
      University NJ 210004, China.
FAU - Cheng, Xue
AU  - Cheng X
AD  - Department of Pathology, Nanjing Maternity and Child Health Hospital, Nanjing 
      Medical University NJ 210004, China.
FAU - Ji, Jie
AU  - Ji J
AD  - Department of Pathology, Nanjing Maternity and Child Health Hospital, Nanjing 
      Medical University NJ 210004, China.
FAU - Zhang, Jingmin
AU  - Zhang J
AD  - Department of Pathology, Nanjing Maternity and Child Health Hospital, Nanjing 
      Medical University NJ 210004, China.
FAU - Zhou, Xiaojun
AU  - Zhou X
AD  - Department of Pathology, Nanjing Jinling Hospital NJ 210002, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20140415
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Case-Control Studies
MH  - ErbB Receptors/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Neoplasm Grading
MH  - *Papanicolaou Test
MH  - Papillomaviridae/isolation & purification
MH  - Phenotype
MH  - Predictive Value of Tests
MH  - Uterine Cervical Neoplasms/*diagnosis/genetics/pathology/virology
MH  - *Vaginal Smears
MH  - Uterine Cervical Dysplasia/*diagnosis/genetics/pathology/virology
PMC - PMC4069959
OTO - NOTNLM
OT  - Cervical intraepithelial lesion
OT  - EGFR
OT  - FISH
OT  - cytogenetics
OT  - gene amplification
EDAT- 2014/06/27 06:00
MHDA- 2015/02/24 06:00
PMCR- 2014/04/15
CRDT- 2014/06/27 06:00
PHST- 2014/01/25 00:00 [received]
PHST- 2014/03/03 00:00 [accepted]
PHST- 2014/06/27 06:00 [entrez]
PHST- 2014/06/27 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2014/04/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2014 Apr 15;7(5):2477-83. eCollection 2014.