PMID- 24967183
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140626
LR  - 20211021
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 7
IP  - 3
DP  - 2014
TI  - PI3K-mediated glioprotective effect of epidermal growth factor under oxidative 
      stress conditions.
PG  - 413-20
LID - 10.3980/j.issn.2222-3959.2014.03.05 [doi]
AB  - AIM: To determine the effects of epidermal growth factor (EGF) on the 
      proliferation and migration of Muller cell line Moorfields/Institute of 
      Ophthalmology-Muller 1 (MIO-M1), and its related molecular mechanisms under 
      normal and oxidative stress conditions. METHODS: Muller cells were cultured with 
      different concentrations of EGF in the presence or absence of varied amounts of 
      H2O2 and glucose oxidase (GO) which induced oxidative stress. The proliferation 
      and migration of Muller cells were examined by 5-Bromo-2-deoxyUridine (BrdU), MTT 
      assay, Transwell assay and scratch wound healing assays. The cell viability was 
      determined with the MTT assay. The secretion of EGF by Muller cells was evaluated 
      by ELISA. Western blot was performed to detect the activation of extracellular 
      regulated protein kinases (ERK)1/2 and Akt signal pathways. RESULTS: EGF 
      stimulated the proliferation and migration of Muller cells in a 
      concentration-dependent manner in vitro. Under oxidative damage condition, 2h of 
      pretreatment with 10-100 ng/mL EGF can mostly inhibit 50% lethal dose of 0.08 
      mmol/L H2O2-induced cell damage. The Western blot results showed that after 
      Muller cells were exposed to varying EGF for 24h, Akt and ERK1/2 were 
      phosphorylated in a dose-dependent manner. In the presence of the LY294002, the 
      potent PI3K inhibitor, the p-Akt was significantly attenuated. CONCLUSION: EGF 
      may induce the proliferation and migration of human Muller cells through the Akt 
      and the ERK1/2 signal pathways, and induce PI3K-mediated glioprotective effect 
      under oxidative stress.
FAU - Hu, Zhi-Xiang
AU  - Hu ZX
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, 
      Wenzhou 325027, Zhejiang Province, China.
FAU - Chen, Chun-Li
AU  - Chen CL
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, 
      Wenzhou 325027, Zhejiang Province, China.
FAU - Yang, Jia-Song
AU  - Yang JS
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, 
      Wenzhou 325027, Zhejiang Province, China.
FAU - Zhou, Zhong-Lou
AU  - Zhou ZL
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, 
      Wenzhou 325027, Zhejiang Province, China.
FAU - Song, Zong-Ming
AU  - Song ZM
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, 
      Wenzhou 325027, Zhejiang Province, China.
FAU - Wang, Zhao-Yang
AU  - Wang ZY
AD  - Department of Ophthalmology, Zhejiang University School of Medicine, Hangzhou 
      310058, Zhejiang Province, China.
LA  - eng
PT  - Journal Article
DEP - 20140618
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC4067651
OTO - NOTNLM
OT  - PI3K
OT  - epidermal growth factor
OT  - human Muller cells
EDAT- 2014/06/27 06:00
MHDA- 2014/06/27 06:01
PMCR- 2014/06/18
CRDT- 2014/06/27 06:00
PHST- 2013/12/21 00:00 [received]
PHST- 2014/02/21 00:00 [accepted]
PHST- 2014/06/27 06:00 [entrez]
PHST- 2014/06/27 06:00 [pubmed]
PHST- 2014/06/27 06:01 [medline]
PHST- 2014/06/18 00:00 [pmc-release]
AID - ijo-07-03-413 [pii]
AID - 10.3980/j.issn.2222-3959.2014.03.05 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2014 Jun 18;7(3):413-20. doi: 
      10.3980/j.issn.2222-3959.2014.03.05. eCollection 2014.