PMID- 24969334
OWN - NLM
STAT- MEDLINE
DCOM- 20141204
LR  - 20140627
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 49
IP  - 4
DP  - 2014 Apr
TI  - [Interaction between HLA-DRB1 gene polymorphism and environmental risk factors in 
      the development of gestational diabetes mellitus].
PG  - 270-5
AB  - OBJECTIVE: To explore the interactions between human leukocyte antigen (HLA) 
      -DRB1 gene polymorphism and environmental risk factors in gestational diabetes 
      mellitus (GDM) pathogenesis. METHODS: Pregnant women who had prenatal cares in 
      Obstetric Department, West China Second Hospital of Sichuan University were 
      recruited from January 1(st) to December 31(st) in 2011. A prospective cohort 
      study was conducted in the women who had a glucose challenge test (GCT) or 75 g 
      oral glucose tolerance test (OGTT) during 24-28 gestational weeks. A total of 104 
      women diagnosed with GDM were randomly included in GDM group while another 103 
      normal women fell into the control group. The HLA-DRB1 polymorphism was detected 
      by Polymerase Chain Reaction - Sequence Specific Primers (PCR-SSP) method in both 
      groups. The interactions between HLA-DRB1 polymorphism and environmental risk 
      factors were analyzed based on the simple-case-study method. RESULTS: (1) There 
      were 712 pregnant women with complete perinatal information during January 1(st) 
      to December 31(st) , 2011, among whom 175 (24.6%) women were diagnosed with GDM. 
      A logistic regression analysis showed that advanced maternal age (OR = 1.081, 
      95%CI: 1.027- 1.138), imbalanced diet (OR = 3.329, 95%CI: 2.167-5.116), high body 
      mass index (BMI >/= 24.0 kg/m(2)) before pregnancy (OR = 1.095, 95%CI:1.008-1.190), 
      HBsAg carrier status (OR = 3.173, 95%CI: 1.387-7.260) and family history of 
      diabetes mellitus (DM) (OR = 1.798, 95%CI: 1.063-3.041) were risk factors of GDM. 
      (2) There were 49 HLA-DRB1 genotypes and 51 HLA-DRB1 genotypes in GDM group and 
      the control group, respectively. We further compared the genotypes that occurred 
      in over 3 cases in either group and found that HLA-DRB1*12, 16 was only detected 
      in 5 cases (5/103, 4.9%) in control group, and the difference was significant 
      between the two groups (P = 0.029). HLA-DRB1*11, 16 and HLA-DRB1*09,09 were only 
      detected in 4 cases (3.8%, 4/104) and 5 cases (4.8%, 5/104) in GDM group 
      respectively, but without significant differences between the two groups (P > 
      0.05). No significant difference was found in other genotype frequencies between 
      the two groups (P > 0.05). (3) Thirteen types of HLA-DRB1 allele were detected 
      but no significant differences were observed in their frequencies between two 
      groups (P > 0.05). (4) A positive interaction was detected between HLA-DRB1*07 
      polymorphism and advanced maternal ages (OR = 5.952, 95%CI:1.314-26.970, P = 
      0.022), while no interaction was found between HLA-DRB polymorphisms to other 
      risk factors such as imbalanced diet, high body mass index (BMI >/= 24.0 kg/m(2)), 
      HBsAg carrier status or DM family history. CONCLUSIONS: Advanced maternal age, 
      unbalanced diet, high body mass index (BMI >/= 24.0 kg/m(2)), HBsAg carrier status 
      and DM family history are environmental risk factors of GDM in Chengdu. While 
      HLA-DRB1*12, 16 genotype may be a protective genotype for GDM. There is a 
      positive interaction between HLA-DRB1*07 polymorphism and advanced maternal age 
      which may play a critic role in GDM development.
FAU - Wang, Ming
AU  - Wang M
AD  - Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan 
      University, Chengdu 610041, China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan 
      University, Chengdu 610041, China. Email: zhanglilxz@163.com.
FAU - Liu, Xinghui
AU  - Liu X
AD  - Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan 
      University, Chengdu 610041, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Body Mass Index
MH  - China/epidemiology
MH  - Diabetes, Gestational/*epidemiology/genetics
MH  - Female
MH  - Gene Frequency
MH  - *Gene-Environment Interaction
MH  - Genotype
MH  - Glucose Tolerance Test
MH  - HLA-DRB1 Chains/*genetics
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - Pregnancy
MH  - Prospective Studies
MH  - Risk Factors
EDAT- 2014/06/28 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/06/28 06:00
PHST- 2014/06/28 06:00 [entrez]
PHST- 2014/06/28 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2014 Apr;49(4):270-5.