PMID- 24969890
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20220321
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 15
IP  - 11
DP  - 2014
TI  - DEPTOR expression negatively correlates with mTORC1 activity and tumor 
      progression in colorectal cancer.
PG  - 4589-94
AB  - The mammalian target of rapamycin (mTOR) signaling pathway is upregulated in the 
      pathogenesis of many cancers, including colorectal cancer (CRC). DEPTOR is an 
      mTOR inhibitor whose expression is negatively regulated by mTOR. However, the 
      role of DEPTOR in the development of CRC is not known. The aim of this study was 
      to investigate the expression of DEPTOR and mTORC1 activity (P-S6) in a subset of 
      CRC patients and determine their relation to tumor differentiation, invasion, 
      nodal metastasis and disease-free survival. Here, Immunohistochemical expression 
      of P-S6 (S235/236) and DEPTOR were evaluated in 1.5 mm tumor cores from 90 CRC 
      patients and in 90 samples of adjacent normal mucosa by tissue microarray. The 
      expression of P-S6 (S235/236) was upregulated in CRC, with the positive rate of 
      P-S6 (S235/236) in CRC (63.3%) significantly higher than that in control tissues 
      (36.7%, 30%) (p<0.05). P-S6 (S235/236) also correlated with high tumor histologic 
      grade (p=0.002), and positive nodal metastasis (p=0.002). In contrast, the 
      expression level of DEPTOR was correlated with low tumor histological grade 
      (p=0.006), and negative nodal metastasis (p=0.001). Interestingly, P-S6 
      (S235/236) expression showed a significant negative association with the 
      expression of DEPTOR in CRC (p=0.011, R= -0.279). However, upregulation of P-S6 
      (S235/236) (p=0.693) and downregulation of DEPTOR (p=0.331) in CRC were not 
      significantly associated with overall survival. Thus, we conclude that expression 
      of DEPTOR negatively correlates with mTORC1 activity and tumor progression in 
      CRC. DEPTOR is a potential marker for prognostic evaluation and a target for the 
      treatment of CRC.
FAU - Lai, Er-Yong
AU  - Lai EY
AD  - The Clinical Research Center for Colorectal Tumor, The Third Affiliated Hospital 
      of Kunming Medical University, Kunming, China E-mail : baixc15@smu.edu.cn.
FAU - Chen, Zhen-Guo
AU  - Chen ZG
FAU - Zhou, Xuan
AU  - Zhou X
FAU - Fan, Xiao-Rong
AU  - Fan XR
FAU - Wang, Hua
AU  - Wang H
FAU - Lai, Ping-Lin
AU  - Lai PL
FAU - Su, Yong-Chun
AU  - Su YC
FAU - Zhang, Bai-Yu
AU  - Zhang BY
FAU - Bai, Xiao-Chun
AU  - Bai XC
FAU - Li, Yun-Feng
AU  - Li YF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Multiprotein Complexes)
RN  - EC 2.7.1.1 (DEPTOR protein, human)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Differentiation/genetics
MH  - Colorectal Neoplasms/*genetics/metabolism/*pathology
MH  - Disease Progression
MH  - Disease-Free Survival
MH  - Down-Regulation/genetics
MH  - Female
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/*genetics
MH  - Lymphatic Metastasis/genetics/pathology
MH  - Male
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Middle Aged
MH  - Multiprotein Complexes/*genetics/*metabolism
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Prognosis
MH  - TOR Serine-Threonine Kinases/*genetics/*metabolism
MH  - Up-Regulation/genetics
MH  - Young Adult
EDAT- 2014/06/28 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/06/28 06:00
PHST- 2014/06/28 06:00 [entrez]
PHST- 2014/06/28 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - 10.7314/apjcp.2014.15.11.4589 [doi]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2014;15(11):4589-94. doi: 10.7314/apjcp.2014.15.11.4589.