PMID- 24970012
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20211021
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 45
IP  - 3
DP  - 2014 Sep
TI  - Phloroglucinol induces apoptosis through the regulation of insulin-like growth 
      factor 1 receptor signaling pathways in human colon cancer HT-29 cells.
PG  - 1036-42
LID - 10.3892/ijo.2014.2521 [doi]
AB  - Phloroglucinol is a polyphenol compound with free radical scavenging, 
      anti-inflammatory and antitumor activity. In this study, we investigated the 
      anticancer effects of phloroglucinol on insulin-like growth factor-1 receptor 
      (IGF-1R) signaling in HT-29 human colon cancer cells. Apoptosis was evaluated 
      using 4',6-diamidino-2-phenylindole (DAPI) staining, which clearly demonstrated 
      cell shrinkage and condensed nuclei. Treatment with a pan-caspase inhibitor 
      reduced the expression of phosphatidylinositol-3-kinase (PI3K)/Akt, which could 
      induce apoptosis through IGF-1R signaling pathways. Treatment with phloroglucinol 
      significantly inhibited the expression of Ras, Raf, mitogen-activated protein 
      kinase (MEK), extracellular-signal regulated kinase (ERK) phosphorylation, PI3K 
      and Akt. Phloroglucinol also decreased mammalian target of rapamycin (mTOR) and 
      expression of its downstream effectors p70S6 kinase and translation initiation 
      factors elF4B and RPS6. These results demonstrate that IGF-1R activates 
      PI3K/Akt/mTOR and Ras/ERK-MAPK downstream signaling pathways, which has important 
      implications for understanding the roles of cell growth pathways in colon cancer 
      cell tumorigenesis.
FAU - Kang, Mi-Hye
AU  - Kang MH
AD  - Departments of Food and Life Science, Pukyong National University, Nam-gu, Busan 
      608-737, Republic of Korea.
FAU - Kim, In-Hye
AU  - Kim IH
AD  - Departments of Food and Life Science, Pukyong National University, Nam-gu, Busan 
      608-737, Republic of Korea.
FAU - Nam, Taek-Jeong
AU  - Nam TJ
AD  - Departments of Food and Life Science, Pukyong National University, Nam-gu, Busan 
      608-737, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140624
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Antineoplastic Agents)
RN  - DHD7FFG6YS (Phloroglucinol)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis
MH  - Cell Cycle/drug effects
MH  - Cell Proliferation
MH  - Colonic Neoplasms/*drug therapy
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - HT29 Cells
MH  - Humans
MH  - Phloroglucinol/*pharmacology
MH  - Receptor, IGF Type 1/genetics/*metabolism
MH  - Signal Transduction/drug effects
PMC - PMC4121399
EDAT- 2014/06/28 06:00
MHDA- 2015/02/24 06:00
PMCR- 2014/06/24
CRDT- 2014/06/28 06:00
PHST- 2014/04/24 00:00 [received]
PHST- 2014/05/28 00:00 [accepted]
PHST- 2014/06/28 06:00 [entrez]
PHST- 2014/06/28 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2014/06/24 00:00 [pmc-release]
AID - ijo-45-03-1036 [pii]
AID - 10.3892/ijo.2014.2521 [doi]
PST - ppublish
SO  - Int J Oncol. 2014 Sep;45(3):1036-42. doi: 10.3892/ijo.2014.2521. Epub 2014 Jun 
      24.