PMID- 24970164
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140627
LR  - 20211021
IS  - 2218-273X (Print)
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 3
IP  - 1
DP  - 2013 Feb 11
TI  - Multi-Color Spectral Transcript Analysis (SPECTRA) for Phenotypic 
      Characterization of Tumor Cells.
PG  - 180-97
LID - 10.3390/biom3010180 [doi]
AB  - Many human tumors show significant changes in their signal transduction pathways 
      and, thus, the way the cells interact with their environment. Often caused by 
      chromosomal rearrangements, including gene amplifications, translocations or 
      deletions, the altered levels of gene expression may provide a tumor-specific 
      signature that can be exploited for diagnostic or therapeutic purposes. We 
      investigated the utility of multiplexed fluorescence in situ hybridization (FISH) 
      using non-isotopically labeled cDNA probes detected by Spectral Imaging as a 
      sensitive and rapid procedure to measure tumor-specific gene expression 
      signatures. We used a commercially available system to acquire and analyze 
      multicolor FISH images. Initial investigations used panels of fluorescent 
      calibration standards to evaluate the system. These experiments were followed by 
      hybridization of five-to-six differently labeled cDNA probes, which target the 
      transcripts of tyrosine kinase genes known to be differently expressed in normal 
      cells and tumors of the breast or thyroid gland. The relatively simple, yet 
      efficient, molecular cytogenetic method presented here may find many applications 
      in characterization of solid tumors or disseminated tumor cells. Addressing tumor 
      heterogeneity by means of multi-parameter single cell analyses is expected to 
      enable a wide range of investigations in the areas of tumor stem cells, tumor 
      clonality and disease progression.
FAU - Hsu, Joanne H
AU  - Hsu JH
AD  - Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 
      98109, USA. jhhsu11@gmail.com.
FAU - Weier, Jingly F
AU  - Weier JF
AD  - Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, 1 Cyclotron 
      Road, Berkeley, CA 94720, USA. jinglyw@gmail.com.
FAU - Weier, Heinz-Ulrich G
AU  - Weier HU
AD  - Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, 1 Cyclotron 
      Road, Berkeley, CA 94720, USA. ugweier@lbl.gov.
FAU - Ito, Yuko
AU  - Ito Y
AD  - National Institute of Science and Technology Policy (NISTEP), Ministry of 
      Education, Culture, Sports, Science and Technology, Tokyo 100-0005, Japan. 
      itoh@nistep.go.jp.
LA  - eng
GR  - R01 CA136685/CA/NCI NIH HHS/United States
GR  - R01 HD045736/HD/NICHD NIH HHS/United States
GR  - R44 HD044313/HD/NICHD NIH HHS/United States
PT  - Journal Article
DEP - 20130211
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
PMC - PMC4030878
EDAT- 2013/01/01 00:00
MHDA- 2013/01/01 00:01
PMCR- 2013/03/01
CRDT- 2014/06/28 06:00
PHST- 2013/01/09 00:00 [received]
PHST- 2013/01/31 00:00 [revised]
PHST- 2013/02/04 00:00 [accepted]
PHST- 2014/06/28 06:00 [entrez]
PHST- 2013/01/01 00:00 [pubmed]
PHST- 2013/01/01 00:01 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - biom3010180 [pii]
AID - biomolecules-03-00180 [pii]
AID - 10.3390/biom3010180 [doi]
PST - epublish
SO  - Biomolecules. 2013 Feb 11;3(1):180-97. doi: 10.3390/biom3010180.