PMID- 24970360
OWN - NLM
STAT- MEDLINE
DCOM- 20150513
LR  - 20211122
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 10
IP  - 3
DP  - 2014 Sep
TI  - Tumor necrosis factor-alpha induces interleukin-34 expression through nuclear 
      factor‑kappaB activation in MC3T3-E1 osteoblastic cells.
PG  - 1371-6
LID - 10.3892/mmr.2014.2353 [doi]
AB  - Osteoblasts produce various types of cytokines under pathological conditions and 
      control osteoclast differentiation. Tumor necrosis factor-alpha (TNF-alpha) has been 
      demonstrated to exert complex effects in osteoblasts under local inflammatory 
      conditions, including in periodontal and periapical diseases. Interleukin-34 
      (IL-34) has been recently identified as a novel regulatory factor for the 
      differentiation and function of osteoclasts. The present study provides the first 
      evidence, to the best of our knowledge, that the expression of IL-34 is induced 
      by TNF-alpha through nuclear factor-kappaB (NF-kappaB) activation in MC3T3-E1 osteoblastic 
      cells. TNF-alpha induced IL-34 expression in a dose- and time-dependent manner. 
      Immunocytochemistry with an NF-kappaB antibody demonstrated that NF-kappaB was mainly 
      localized in the cytoplasm of the untreated MC3T3-E1 cells. Rapid translocation 
      of NF-kappaB from the cytoplasm to the nucleus was observed in the cells treated with 
      TNF-alpha for 15 min. Translocation and transcriptional activity of NF-kappaB were also 
      determined by western blotting and a luciferase reporter assay, respectively. 
      Pretreatment with 100 microM CAPE, an inhibitor of NF-kappaB, significantly inhibited 
      TNF-alpha-induced IL-34 expression. These results indicate that TNF-alpha induces IL-34 
      expression via NF-kappaB in osteoblasts.
FAU - Yu, Yaqiong
AU  - Yu Y
AD  - Department of Endodontics, School of Stomatology, China Medical University, 
      Shenyang, Liaoning 110002, P.R. China.
FAU - Yang, Di
AU  - Yang D
AD  - Department of Endodontics, School of Stomatology, China Medical University, 
      Shenyang, Liaoning 110002, P.R. China.
FAU - Qiu, Lihong
AU  - Qiu L
AD  - Department of Endodontics, School of Stomatology, China Medical University, 
      Shenyang, Liaoning 110002, P.R. China.
FAU - Okamura, Hirohiko
AU  - Okamura H
AD  - Department of Histology and Oral Histology, Institute of Health Biosciences, The 
      University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan.
FAU - Guo, Jiajie
AU  - Guo J
AD  - Department of Endodontics, School of Stomatology, China Medical University, 
      Shenyang, Liaoning 110002, P.R. China.
FAU - Haneji, Tatsuji
AU  - Haneji T
AD  - Department of Histology and Oral Histology, Institute of Health Biosciences, The 
      University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan.
LA  - eng
PT  - Journal Article
DEP - 20140625
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Interleukins)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (interleukin-34, mouse)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Cell Nucleus/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Interleukins/genetics/*metabolism
MH  - Mice
MH  - NF-kappa B/genetics/*metabolism
MH  - Osteoblasts/cytology/*drug effects/metabolism
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/*pharmacology
PMC - PMC4121411
EDAT- 2014/06/28 06:00
MHDA- 2015/05/15 06:00
PMCR- 2014/06/25
CRDT- 2014/06/28 06:00
PHST- 2013/10/10 00:00 [received]
PHST- 2014/04/24 00:00 [accepted]
PHST- 2014/06/28 06:00 [entrez]
PHST- 2014/06/28 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
PHST- 2014/06/25 00:00 [pmc-release]
AID - mmr-10-03-1371 [pii]
AID - 10.3892/mmr.2014.2353 [doi]
PST - ppublish
SO  - Mol Med Rep. 2014 Sep;10(3):1371-6. doi: 10.3892/mmr.2014.2353. Epub 2014 Jun 25.