PMID- 24971649
OWN - NLM
STAT- MEDLINE
DCOM- 20150819
LR  - 20140805
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 86
IP  - 15
DP  - 2014 Aug 5
TI  - Self-phosphorylating deoxyribozyme initiated cascade enzymatic amplification for 
      guanosine-5'-triphosphate detection.
PG  - 7907-12
LID - 10.1021/ac501842t [doi]
AB  - The self-phosphorylating deoxyribozymes identified by in vitro selection can 
      catalyze their own phosphorylation by utilizing phosphate donor 
      guanosine-5'-triphosphate (GTP) which plays a critical role in a majority of 
      cellular processes. On the basis of the unique properties of self-phosphorylating 
      deoxyribozymes, we report a novel GTP sensor coupled with lambda exonuclease cleavage 
      reaction and nicking enzyme assisted fluorescence signal amplification process. 
      The deoxyribozymes with special catalytic and structural characteristics display 
      good stability compared to protein and RNA enzymes. We combined these properties 
      with enzymatic recycling cleavage strategy to build a sensor which produced 
      enhanced fluorescence signal. Sensitive and selective detection of GTP was 
      successfully realized with the well-designed deoxyribozyme-based sensing platform 
      by taking advantage of the self-phosphorylating ability of the kinase 
      deoxyribozyme, efficient digestion capacity of lambda exonuclease, and enzymatic 
      recycling amplification of nicking enzyme. The method not only provides a 
      platform for detecting GTP but also shows great potential in analyzing a variety 
      of targets by combining deoxyribozymes with signal amplification strategy.
FAU - Wang, Lida
AU  - Wang L
AD  - Department of Chemistry, Beijing Key Laboratory for Analytical Methods and 
      Instrumentation, Tsinghua University , Beijing 100084, China.
FAU - Liu, Yang
AU  - Liu Y
FAU - Li, Jinghong
AU  - Li J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140710
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (DNA, Catalytic)
RN  - 86-01-1 (Guanosine Triphosphate)
SB  - IM
MH  - DNA, Catalytic/*metabolism
MH  - Guanosine Triphosphate/*analysis
MH  - Limit of Detection
MH  - Phosphorylation
EDAT- 2014/06/28 06:00
MHDA- 2015/08/20 06:00
CRDT- 2014/06/28 06:00
PHST- 2014/06/28 06:00 [entrez]
PHST- 2014/06/28 06:00 [pubmed]
PHST- 2015/08/20 06:00 [medline]
AID - 10.1021/ac501842t [doi]
PST - ppublish
SO  - Anal Chem. 2014 Aug 5;86(15):7907-12. doi: 10.1021/ac501842t. Epub 2014 Jul 10.