PMID- 24972136
OWN - NLM
STAT- MEDLINE
DCOM- 20150224
LR  - 20211021
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 15
IP  - 7
DP  - 2014 Jun 26
TI  - Genetic variants of APOC3 promoter and HLA-B genes in an HIV infected cohort in 
      northern South Africa: a pilot study.
PG  - 11403-15
LID - 10.3390/ijms150711403 [doi]
AB  - Metabolic disorders and hypersensitivities affect tolerability and impact 
      adherence to highly active antiretroviral therapy (HAART). The aim of this study 
      was to determine the prevalence of C-482T/T-455C variants in the Apolipoprotein 
      C3 (APOC3) promoter gene and Human leukocyte antigen (HLA)-B*57:01, known to 
      impact lipid metabolic disorders and hypersensitivity respectively; and to 
      correlate genotypes with gender, CD4+ cell count and viral load in an HIV 
      infected cohort in northern South Africa. Frequencies of C-482 and T-455 
      polymorphisms in APOC3 were determined by restriction fragment length 
      polymorphism analysis. Allele determination for HLA-B was performed with Assign 
      SBT software in an HLA library. Analysis of APOC3 C-482 site revealed a 
      prevalence of 196/199 (98.5%) for CC, 1/199 (0.5%) for CT and 2/199 (1.0%) for TT 
      genotype (p = 0.000 with 1 degrees  of freedom; chi2 = 126.551). For the T-455 site, 
      prevalences were: 69/199 (35%) for TT and 130/199 (65%) for the CC genotype (p = 
      0.000 with 1 degrees  of freedom; chi2 = 199). There was no association between gender and 
      the presence of -482 (p = 1; chi2 = 0.00001) or -455 genotypes (p = 0.1628; chi2 = 
      1.9842). There was no significant difference in the increase in CD4+ cell count 
      irrespective of genotypes. Significant increases in CD4+ cell count were observed 
      in males and females considering the -455C genotype, but not in males for the 
      -455T genotype. Viral load decreases were significant with the -455C and -482C 
      genotypes irrespective of gender. HLA-B*57:01 was not identified in the study 
      cohort. The apparently high prevalence of APOC3 T-455CC genotype needs 
      confirmation with a larger samples size and triglyceride measurements to support 
      screening of patients to pre-empt HAART associated lipid disorders.
FAU - Masebe, Tracy
AU  - Masebe T
AD  - HIV/AIDS & Global Health Research Programme, Department of Microbiology, 
      University of Venda, Thohoyandou 0950, South Africa. tracy.masebe@univen.ac.za.
FAU - Bessong, Pascal Obong
AU  - Bessong PO
AD  - HIV/AIDS & Global Health Research Programme, Department of Microbiology, 
      University of Venda, Thohoyandou 0950, South Africa. bessong@univen.ac.za.
FAU - Ndip, Roland Ndip
AU  - Ndip RN
AD  - Department of Microbiology and Parasitology, Faculty of Science, University of 
      Buea, Buea Box 63, Cameroon. ndip3@yahoo.com.
FAU - Meyer, Debra
AU  - Meyer D
AD  - Department of Biochemistry, University of Pretoria, Pretoria 0002, South Africa. 
      debra.meyer@up.ac.za.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140626
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Apolipoproteins C)
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Apolipoproteins C/*genetics
MH  - CD4 Lymphocyte Count
MH  - Female
MH  - HIV Infections/*genetics
MH  - HLA-B Antigens/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - *Promoter Regions, Genetic
MH  - South Africa
PMC - PMC4139789
EDAT- 2014/06/28 06:00
MHDA- 2015/02/25 06:00
PMCR- 2014/07/01
CRDT- 2014/06/28 06:00
PHST- 2013/12/28 00:00 [received]
PHST- 2014/03/17 00:00 [revised]
PHST- 2014/04/11 00:00 [accepted]
PHST- 2014/06/28 06:00 [entrez]
PHST- 2014/06/28 06:00 [pubmed]
PHST- 2015/02/25 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - ijms150711403 [pii]
AID - ijms-15-11403 [pii]
AID - 10.3390/ijms150711403 [doi]
PST - epublish
SO  - Int J Mol Sci. 2014 Jun 26;15(7):11403-15. doi: 10.3390/ijms150711403.