PMID- 24973032
OWN - NLM
STAT- MEDLINE
DCOM- 20150122
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1355
DP  - 2014 Aug 15
TI  - One-pot preparation of glutathione-silica hybrid monolith for mixed-mode 
      capillary liquid chromatography based on "thiol-ene" click chemistry.
PG  - 228-37
LID - S0021-9673(14)00920-0 [pii]
LID - 10.1016/j.chroma.2014.06.023 [doi]
AB  - A novel glutathione (GSH)-silica hybrid monolithic column synthesized via a 
      combination of thiol-ene click reaction and one-pot process was described, where 
      thiol-end GSH organic monomer and 2,2-azobisisobutyronitrile (AIBN) were mixed 
      with hydrolyzed tetramethyloxysilane (TMOS) and 
      gamma-methacryloxypropyltrimethoxysilane (gamma-MAPS) and then introduced into a 
      fused-silica capillary for simultaneous polycondensation and "thiol-ene" click 
      reaction to form the GSH-silica hybrid monolith. The effects of the molar ratio 
      of TMOS/gamma-MAPS, the amount of GSH, and the volume of porogen on the morphology, 
      permeability and pore properties of the prepared GSH-silica hybrid monoliths were 
      studied in detail. A uniform monolithic network with high porosity was obtained. 
      A series of test compounds including alkylbenzenes, amides, and anilines were 
      used to evaluate the retention behaviors of the GSH-silica hybrid monolithic 
      column. The results demonstrated that the prepared GSH-silica hybrid monolith 
      exhibited multiple interactions including hydrophobicity, hydrophilicity, as well 
      as cation exchange interaction. The run-to-run, column-to-column and 
      batch-to-batch reproducibilities of the GSH-silica hybrid monolith for phenols' 
      retention were satisfactory with the relative standard deviations (RSDs) less 
      than 1.3% (n=5), 2.6% (n=3) and 3.2% (n=3), respectively, indicating the 
      effectiveness and practicability of the proposed method. In addition, the 
      GSH-silica hybrid monolith was applied to the separation of nucleotides, peptides 
      and protein tryptic digests, respectively. The successful applications suggested 
      the potential of the GSH-silica hybrid monolith in complex sample analysis.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Lin, Zian
AU  - Lin Z
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food 
      Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, Fujian, China. 
      Electronic address: zianlin@fzu.edu.cn.
FAU - Tan, Xiaoqing
AU  - Tan X
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food 
      Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, Fujian, China.
FAU - Yu, Ruifang
AU  - Yu R
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food 
      Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, Fujian, China.
FAU - Lin, Jiashi
AU  - Lin J
AD  - College of Physical Education, Jimei University, Xiamen 361021, China.
FAU - Yin, Xiaofei
AU  - Yin X
AD  - The First Institute of Oceanography, SOA, Qingdao 266061, China.
FAU - Zhang, Lan
AU  - Zhang L
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food 
      Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, Fujian, China. 
      Electronic address: zlan@fzu.edu.cn.
FAU - Yang, Huanghao
AU  - Yang H
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food 
      Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, Fujian, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140612
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Methacrylates)
RN  - 0 (Peptides)
RN  - 0 (Proteins)
RN  - 0 (Silanes)
RN  - 0 (Sulfhydryl Compounds)
RN  - 2530-85-0 (methacryloxypropyltrimethoxysilane)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Click Chemistry/instrumentation/*methods
MH  - Glutathione/*chemistry
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Methacrylates/chemistry
MH  - Microscopy, Electron, Scanning
MH  - Peptides/isolation & purification
MH  - Proteins/isolation & purification
MH  - Silanes/chemistry
MH  - Silicon Dioxide/*chemistry
MH  - Sulfhydryl Compounds/chemistry
OTO - NOTNLM
OT  - Capillary liquid chromatography
OT  - Click chemistry
OT  - Glutathione
OT  - Mixed-mode
OT  - One-pot
OT  - Organic-inorganic hybrid monolith
EDAT- 2014/06/29 06:00
MHDA- 2015/01/23 06:00
CRDT- 2014/06/29 06:00
PHST- 2014/03/21 00:00 [received]
PHST- 2014/06/03 00:00 [revised]
PHST- 2014/06/05 00:00 [accepted]
PHST- 2014/06/29 06:00 [entrez]
PHST- 2014/06/29 06:00 [pubmed]
PHST- 2015/01/23 06:00 [medline]
AID - S0021-9673(14)00920-0 [pii]
AID - 10.1016/j.chroma.2014.06.023 [doi]
PST - ppublish
SO  - J Chromatogr A. 2014 Aug 15;1355:228-37. doi: 10.1016/j.chroma.2014.06.023. Epub 
      2014 Jun 12.