PMID- 24973071
OWN - NLM
STAT- MEDLINE
DCOM- 20150414
LR  - 20211021
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 80
IP  - 17
DP  - 2014 Sep
TI  - Ostreid herpesvirus 1 infection among Pacific oyster (Crassostrea gigas) Spat: 
      relevance of water temperature to virus replication and circulation prior to the 
      onset of mortality.
PG  - 5419-26
LID - 10.1128/AEM.00484-14 [doi]
AB  - A number of bivalve species worldwide, including the Pacific oyster, Crassostrea 
      gigas, have been affected by mass mortality events associated with herpesviruses, 
      resulting in significant losses. A particular herpesvirus was purified from 
      naturally infected larval Pacific oysters, and its genome was completely 
      sequenced. This virus has been classified as Ostreid herpesvirus 1 (OsHV-1) 
      within the family Malacoherpesviridae. Since 2008, mass mortality outbreaks among 
      C. gigas in Europe have been related to the detection of a variant of OsHV-1 
      called muVar. Additional data are necessary to better describe mortality events in 
      relation to environmental-parameter fluctuations and OsHV-1 detection. For this 
      purpose, a single batch of Pacific oyster spat was deployed in 4 different 
      locations in the Marennes-Oleron area (France): an oyster pond ("claire"), a 
      shellfish nursery, and two locations in the field. Mortality rates were recorded 
      based on regular observation, and samples were collected to search for and 
      quantify OsHV-1 DNA by real-time PCR. Although similar massive mortality rates 
      were reported at the 4 sites, mortality was detected earlier in the pond and in 
      the nursery than at both field sites. This difference may be related to earlier 
      increases in water temperature. Mass mortality was observed among oysters a few 
      days after increases in the number of PCR-positive oysters and viral-DNA amounts 
      were recorded. An initial increment in the number of PCR-positive oysters was 
      reported at both field sites during the survey in the absence of significant 
      mortality. During this period, the water temperature was below 16 degrees C.
CI  - Copyright (c) 2014, American Society for Microbiology. All Rights Reserved.
FAU - Renault, Tristan
AU  - Renault T
AD  - Ifremer, Unite Sante Genetique et Microbiologie des Mollusques (SG2M), 
      Laboratoire de Genetique et Pathologie des Mollusques Marins (LGPMM), La 
      Tremblade, France Tristan.Renault@ifremer.fr.
FAU - Bouquet, Anne Lise
AU  - Bouquet AL
AD  - Centre Regional Experimentation et Application Aquacole (CREAA), Prise de 
      Terdoux, Le Chateau d'Oleron, France.
FAU - Maurice, Julien-Thomas
AU  - Maurice JT
AD  - Centre Regional Experimentation et Application Aquacole (CREAA), Prise de 
      Terdoux, Le Chateau d'Oleron, France.
FAU - Lupo, Coralie
AU  - Lupo C
AD  - Ifremer, Unite Sante Genetique et Microbiologie des Mollusques (SG2M), 
      Laboratoire de Genetique et Pathologie des Mollusques Marins (LGPMM), La 
      Tremblade, France.
FAU - Blachier, Philippe
AU  - Blachier P
AD  - Centre Regional Experimentation et Application Aquacole (CREAA), Prise de 
      Terdoux, Le Chateau d'Oleron, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140627
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Viral)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Animals
MH  - Crassostrea/*virology
MH  - DNA, Viral/analysis/genetics/isolation & purification
MH  - France
MH  - Herpesviridae/*isolation & purification/*physiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Survival Analysis
MH  - Temperature
MH  - Viral Load
MH  - *Virus Replication
MH  - Water
PMC - PMC4136091
EDAT- 2014/06/29 06:00
MHDA- 2015/04/15 06:00
PMCR- 2015/03/01
CRDT- 2014/06/29 06:00
PHST- 2014/06/29 06:00 [entrez]
PHST- 2014/06/29 06:00 [pubmed]
PHST- 2015/04/15 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - AEM.00484-14 [pii]
AID - 00484-14 [pii]
AID - 10.1128/AEM.00484-14 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2014 Sep;80(17):5419-26. doi: 10.1128/AEM.00484-14. Epub 
      2014 Jun 27.