PMID- 24973771 OWN - NLM STAT- MEDLINE DCOM- 20150330 LR - 20140728 IS - 1873-376X (Electronic) IS - 1570-0232 (Linking) VI - 965 DP - 2014 Aug 15 TI - A quantitative, selective and fast ultra-high performance liquid chromatography tandem mass spectrometry method for the simultaneous analysis of 33 basic drugs in hair (amphetamines, cocaine, opiates, opioids and metabolites). PG - 7-18 LID - S1570-0232(14)00371-7 [pii] LID - 10.1016/j.jchromb.2014.05.055 [doi] AB - Forensic testing for drugs of abuse in hair has become a useful diagnostic tool in determining chronic drug use as well as examining long-term drug history thorough segmental analysis. However, sensitive and specific analytical methods are needed. A simple, rapid and highly sensitive and specific method for the extraction and quantification of 33 opioids, opiates, cocaine, and amphetamines is presented. The method was fully validated according to international guidelines. Twenty milligrams of hair sample was pulverized and then incubated in the same disposable tube with methanol (under sonication at 45 degrees C) during 4h. After centrifugation the supernatant was evaporated up to about 100 muL and a solid phase extraction (SPE) followed by separation and quantification using ultra performance liquid chromatography-tandem mass spectrometry (UHLC-MS/MS) were carried out. Chromatographic separation was achieved using a BEH phenyl column eluted with 0.1% formic acid: methanol (0.1% formic acid). Selectivity of the method was achieved by a combination of retention time, and two precursor-product ion transitions. Good intra-assay and inter-assay precision (relative standard deviations (RSDs) were observed (<15%) for most of the compounds. The lower limit of quantification was fixed at the lowest calibrator in the linearity experiments and it ranged from 0.006 to 0.063 ng/mg. No instability was observed in processed samples. Extraction efficiency varied from 37 to 107% (except for EDDP with a recovery of 5%) and matrix effects ranged from 52 to 160%, and for most of the compounds it was compensated by the internal standard (IS). The method was subsequently applied to authentic hair samples obtained from forensic and toxicology cases and to proficiency test (obtaining z-scores lower than 1 for most of the compounds). The validation and actual sample analysis results show that this method is rugged, precise, accurate, and well-suited for routine hair analysis. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Fernandez, Maria Del Mar Ramirez AU - Fernandez Mdel M AD - Federal Public Service Justice, National Institute of Criminalistics and Criminology, Brussels, Belgium. Electronic address: mariadelmar.ramirezfernandez@just.fgov.be. FAU - Di Fazio, Vincent AU - Di Fazio V AD - Federal Public Service Justice, National Institute of Criminalistics and Criminology, Brussels, Belgium. FAU - Wille, Sarah M R AU - Wille SM AD - Federal Public Service Justice, National Institute of Criminalistics and Criminology, Brussels, Belgium. FAU - Kummer, Natalie AU - Kummer N AD - Federal Public Service Justice, National Institute of Criminalistics and Criminology, Brussels, Belgium. FAU - Samyn, Nele AU - Samyn N AD - Federal Public Service Justice, National Institute of Criminalistics and Criminology, Brussels, Belgium. LA - eng PT - Journal Article DEP - 20140606 PL - Netherlands TA - J Chromatogr B Analyt Technol Biomed Life Sci JT - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JID - 101139554 RN - 0 (Amphetamines) RN - 0 (Analgesics, Opioid) RN - I5Y540LHVR (Cocaine) SB - IM MH - Adult MH - Amphetamines/*analysis/chemistry/isolation & purification MH - Analgesics, Opioid/*analysis/chemistry/isolation & purification MH - Chromatography, High Pressure Liquid/*methods MH - Cocaine/*analysis/chemistry/isolation & purification MH - Hair/*chemistry MH - Humans MH - Linear Models MH - Male MH - Reproducibility of Results MH - Sensitivity and Specificity MH - Tandem Mass Spectrometry/*methods OTO - NOTNLM OT - Amphetamines OT - Cocaine OT - Hair OT - Opioids OT - Proficiency test OT - UPLC-MS/MS EDAT- 2014/06/29 06:00 MHDA- 2015/03/31 06:00 CRDT- 2014/06/29 06:00 PHST- 2014/02/25 00:00 [received] PHST- 2014/05/27 00:00 [revised] PHST- 2014/05/31 00:00 [accepted] PHST- 2014/06/29 06:00 [entrez] PHST- 2014/06/29 06:00 [pubmed] PHST- 2015/03/31 06:00 [medline] AID - S1570-0232(14)00371-7 [pii] AID - 10.1016/j.jchromb.2014.05.055 [doi] PST - ppublish SO - J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Aug 15;965:7-18. doi: 10.1016/j.jchromb.2014.05.055. Epub 2014 Jun 6.