PMID- 24974031
OWN - NLM
STAT- MEDLINE
DCOM- 20150213
LR  - 20140630
IS  - 0091-679X (Print)
IS  - 0091-679X (Linking)
VI  - 123
DP  - 2014
TI  - Nanoscale cellular imaging with scanning angle interference microscopy.
PG  - 235-52
LID - B978-0-12-420138-5.00013-6 [pii]
LID - 10.1016/B978-0-12-420138-5.00013-6 [doi]
AB  - Fluorescence microscopy is among the most widely utilized tools in cell and 
      molecular biology due to its ability to noninvasively obtain time-resolved images 
      of live cells with molecule-specific contrast. In this chapter, we describe a 
      simple high-resolution technique, scanning angle interference microscopy (SAIM), 
      for the imaging and localization of fluorescent molecules with nanometer 
      precision along the optical axis. In SAIM, samples above a reflective surface are 
      sequentially scanned with an excitation laser at varying angles of incidence. 
      Interference patterns generated between the incident and reflected lights result 
      in an emission intensity that depends on the height of a fluorophore above the 
      silicon surface and the angle of the incident radiation. The measured 
      fluorescence intensities are then fit to an optical model to localize the labeled 
      molecules along the z-axis with 5-10 nm precision and diffraction-limited lateral 
      resolution. SAIM is easily implemented on widely available commercial total 
      internal reflection fluorescence microscopes, offering potential for widespread 
      use in cell biology. Here, we describe the setup of SAIM and its application for 
      imaging cellular structures near (<1 mum) the sample substrate.
CI  - (c) 2014 Elsevier Inc. All rights reserved.
FAU - DuFort, Christopher
AU  - DuFort C
AD  - Department of Surgery, University of California, San Francisco, California, USA; 
      Department of Orthopaedic Surgery, University of California, San Francisco, 
      California, USA.
FAU - Paszek, Matthew
AU  - Paszek M
AD  - School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New 
      York, USA; Kavli Institute at Cornell for Nanoscale Science, Ithaca, New York, 
      USA.
LA  - eng
GR  - U54CA143836-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Cell Biol
JT  - Methods in cell biology
JID - 0373334
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Cell Line
MH  - Fluorescent Dyes/chemistry
MH  - Focal Adhesions/ultrastructure
MH  - Humans
MH  - Microscopy, Fluorescence/methods
MH  - Microscopy, Interference/methods
MH  - Nanotechnology
MH  - Single-Cell Analysis/*methods
MH  - Staining and Labeling
OTO - NOTNLM
OT  - Cytoskeleton
OT  - Dynamic
OT  - Focal adhesion
OT  - Interference
OT  - Membrane
OT  - Microscopy
OT  - Nanoscale
OT  - Superresolution
EDAT- 2014/06/30 06:00
MHDA- 2015/02/14 06:00
CRDT- 2014/06/30 06:00
PHST- 2014/06/30 06:00 [entrez]
PHST- 2014/06/30 06:00 [pubmed]
PHST- 2015/02/14 06:00 [medline]
AID - B978-0-12-420138-5.00013-6 [pii]
AID - 10.1016/B978-0-12-420138-5.00013-6 [doi]
PST - ppublish
SO  - Methods Cell Biol. 2014;123:235-52. doi: 10.1016/B978-0-12-420138-5.00013-6.