PMID- 24975446 OWN - NLM STAT- MEDLINE DCOM- 20150413 LR - 20161125 IS - 1872-7077 (Electronic) IS - 1382-6689 (Linking) VI - 38 IP - 1 DP - 2014 Jul TI - Effects of ginsenoside Rb1 on the stress-induced changes of BDNF and HSP70 expression in rat hippocampus. PG - 257-62 LID - S1382-6689(14)00147-1 [pii] LID - 10.1016/j.etap.2014.06.004 [doi] AB - Ginsenoside Rb1 (GRb1) has been determined to exert diverse neuromodulatory effects including antistress effects in the brain. The hippocampus is a key brain structure for memory, learning, and cognition and is especially vulnerable to neurotoxic effects associated with stress. The aim of this study was to further explore neuroprotective potential of GRb1 on stress-mediated changes in hippocampal gene expression. Recent studies recognize agents that inducing brain-derived neurotrophic factor (BDNF) and heat shock protein (HSP) 70 as important neuroprotective approaches. Thus, we specifically determined the effects of GRb1 on mRNA expression of BDNF and HSP70, in a model of immobilization stress. In agreement with these reports, acute immobilization stress led to a decrease and an increase in the mRNA levels of the BDNF and HSP70, respectively, in the hippocampus. When pretreated orally, GRb1 significantly inhibited the stress-mediated decline of BDNF level whereas it further increased the stress-mediated elevation of HSP70 level. Our results strongly suggest GRb1 effective in controlling stress-related hippocampal dysfunction. Our finding also contributes further understanding of medicinal usefulness of GRb1 targeting hippocampal network alteration which is commonly observed in aging and neurodegenerative disorders. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Kim, Mia AU - Kim M AD - Department of Cardiovascular & Neurologic Diseases (Stroke Center), Hospital of Oriental Medicine, Kyung Hee University, Seoul 130-702, Republic of Korea. FAU - Kim, Sung-Ok AU - Kim SO AD - College of Oriental Medicine, Daegu Haany University, Daegu 706-060, Republic of Korea. FAU - Lee, Moonsung AU - Lee M AD - Department of East-West Medical Science, Kyung Hee University, Yongin-si 446-701, Republic of Korea. FAU - Park, Yeri AU - Park Y AD - Department of East-West Medical Science, Kyung Hee University, Yongin-si 446-701, Republic of Korea. FAU - Kim, Danhyo AU - Kim D AD - Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si 446-701, Republic of Korea. FAU - Cho, Ki-Ho AU - Cho KH AD - Department of Cardiovascular & Neurologic Diseases (Stroke Center), Hospital of Oriental Medicine, Kyung Hee University, Seoul 130-702, Republic of Korea. FAU - Kim, Sun Yeou AU - Kim SY AD - College of Pharmacy, Gachon University, Incheon 406-799, Republic of Korea. FAU - Lee, Eunjoo H AU - Lee EH AD - Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si 446-701, Republic of Korea. Electronic address: ehwang@khu.ac.kr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140620 PL - Netherlands TA - Environ Toxicol Pharmacol JT - Environmental toxicology and pharmacology JID - 9612020 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Ginsenosides) RN - 0 (HSP70 Heat-Shock Proteins) RN - 0 (Neuroprotective Agents) RN - 0 (RNA, Messenger) RN - 7413S0WMH6 (ginsenoside Rb1) RN - W980KJ009P (Corticosterone) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*genetics MH - Corticosterone/blood MH - Gene Expression Regulation/drug effects MH - Ginsenosides/*pharmacology MH - HSP70 Heat-Shock Proteins/*genetics/metabolism MH - Hippocampus/*drug effects/metabolism MH - Male MH - Neuroprotective Agents/*pharmacology MH - RNA, Messenger/metabolism MH - Rats, Sprague-Dawley MH - Restraint, Physical MH - Stress, Psychological/*genetics/metabolism OTO - NOTNLM OT - Brain-derived neurotrophic factor OT - Ginsenoside Rb1 OT - Heat shock protein 70 OT - Hippocampus OT - Immobilization stress OT - Real-time PCR EDAT- 2014/07/01 06:00 MHDA- 2015/04/14 06:00 CRDT- 2014/07/01 06:00 PHST- 2013/07/09 00:00 [received] PHST- 2014/06/06 00:00 [revised] PHST- 2014/06/11 00:00 [accepted] PHST- 2014/07/01 06:00 [entrez] PHST- 2014/07/01 06:00 [pubmed] PHST- 2015/04/14 06:00 [medline] AID - S1382-6689(14)00147-1 [pii] AID - 10.1016/j.etap.2014.06.004 [doi] PST - ppublish SO - Environ Toxicol Pharmacol. 2014 Jul;38(1):257-62. doi: 10.1016/j.etap.2014.06.004. Epub 2014 Jun 20.