PMID- 24979738
OWN - NLM
STAT- MEDLINE
DCOM- 20150710
LR  - 20141202
IS  - 1089-8646 (Electronic)
IS  - 0888-7543 (Linking)
VI  - 104
IP  - 4
DP  - 2014 Oct
TI  - Integrated transcriptome analysis reveals miRNA-mRNA crosstalk in laryngeal 
      squamous cell carcinoma.
PG  - 249-56
LID - S0888-7543(14)00097-4 [pii]
LID - 10.1016/j.ygeno.2014.06.004 [doi]
AB  - Next generation sequencing (NGS) has proven to be a powerful tool in delineating 
      myriads of molecular subtypes of cancer, as well as in revealing accumulation of 
      genomic mutations throughout cancer progression. Whole genome microRNA (miRNA) 
      and mRNA expression profiles were obtained from patients with laryngeal squamous 
      cell carcinoma (LSCC) using deep sequencing technology, and were analyzed by 
      utilizing integrative computational approaches. A large number of protein-coding 
      and non-coding genes were detected to be differentially expressed, indicating a 
      functional switch in LSCC cells. A total of 127 mutated genes were detected to be 
      significantly associated with ectoderm and epidermis development. Eleven miRNAs 
      were found to be differentially expressed, including a potential cancer 
      suppressor miRNA, mir-34c, which was dramatically down-regulated. Integrated 
      analysis of mRNA and miRNA transcriptomes further revealed correlated dynamics 
      among 11 miRNAs and 138 targeted genes, forming a highly dynamical co-regulation 
      network response to LSCC development.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Zhang, Yang
AU  - Zhang Y
AD  - Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, 
      Capital Medical University, Key Laboratory of Otolaryngology Head and Neck 
      Surgery, Beijing 100005, China.
FAU - Chen, Yong
AU  - Chen Y
AD  - National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Yu, Jinhai
AU  - Yu J
AD  - National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Liu, Guiming
AU  - Liu G
AD  - Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, 
      Chinese Academy of Sciences, Beijing 100101, China. Electronic address: 
      liugm@big.ac.cn.
FAU - Huang, Zhigang
AU  - Huang Z
AD  - Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, 
      Capital Medical University, Key Laboratory of Otolaryngology Head and Neck 
      Surgery, Beijing 100005, China. Electronic address: huangzhigangent@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140628
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - DNA, Mitochondrial/genetics
MH  - Down-Regulation
MH  - Gene Expression Regulation, Neoplastic
MH  - *Gene Regulatory Networks
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - MicroRNAs/*genetics
MH  - Mutation Rate
MH  - RNA, Messenger/*genetics
MH  - Sequence Analysis, DNA
MH  - *Transcriptome
OTO - NOTNLM
OT  - Data integration
OT  - Gene expression
OT  - Laryngeal squamous cell carcinoma
OT  - Small RNAs
EDAT- 2014/07/01 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/07/01 06:00
PHST- 2014/04/30 00:00 [received]
PHST- 2014/06/19 00:00 [revised]
PHST- 2014/06/23 00:00 [accepted]
PHST- 2014/07/01 06:00 [entrez]
PHST- 2014/07/01 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - S0888-7543(14)00097-4 [pii]
AID - 10.1016/j.ygeno.2014.06.004 [doi]
PST - ppublish
SO  - Genomics. 2014 Oct;104(4):249-56. doi: 10.1016/j.ygeno.2014.06.004. Epub 2014 Jun 
      28.