PMID- 24982218
OWN - NLM
STAT- MEDLINE
DCOM- 20150220
LR  - 20140701
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Linking)
VI  - 28
IP  - 4
DP  - 2014 Jul-Aug
TI  - Determination of the LD50 of acridine orange via intravenous administration in 
      mice in preparation for clinical application to cancer therapy.
PG  - 523-7
AB  - AIM: We undertook studies to determine the lethal dose 50 (LD50) of acridine 
      orange (AO) using mice in order to confirm the safety of intravenous 
      administration of AO. MATERIALS AND METHODS: We used 40 mice and AO was 
      administered once intravenously. General behavior and mortality were continuously 
      observed for 14 days. At the end of the experiment, all animals were sacrificed 
      for subsequent studies. RESULTS: The LD50 for AO in male and female mice was 
      determined to be 32 mg/kg and 36 mg/kg, respectively. Histopathological 
      abnormalities were observed in only one mouse which died three days after the 
      administration of AO. The other nine mice which died immediately after the 
      administration of AO had no pathological findings in major organs. CONCLUSION: 
      The clinical use of AO can be kept at 1.0 mg/kg or below and, therefore, 
      intravenous administration of AO might be safe for use as cancer therapy.
CI  - Copyright (c) 2014 International Institute of Anticancer Research (Dr. John G. 
      Delinassios), All rights reserved.
FAU - Nakamura, Tomoki
AU  - Nakamura T
AD  - Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 
      Mie, Japan tomoki66@clin.medic.mie-u.ac.jp.
FAU - Kusuzaki, Katsuyuki
AU  - Kusuzaki K
AD  - Department of Orthopaedic Surgery, Kujyo Hospital, Kyoto, Japan.
FAU - Matsubara, Takao
AU  - Matsubara T
AD  - Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 
      Mie, Japan.
FAU - Matsumine, Akihiko
AU  - Matsumine A
AD  - Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 
      Mie, Japan.
FAU - Asanuma, Kunihiro
AU  - Asanuma K
AD  - Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 
      Mie, Japan.
FAU - Satonaka, Haruhiko
AU  - Satonaka H
AD  - Department of Orthopaedic Surgery, Ise Municipal General Hospital, Ise, Japan.
FAU - Uchida, Atsumasa
AU  - Uchida A
AD  - Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 
      Mie, Japan.
FAU - Sudo, Akihiro
AU  - Sudo A
AD  - Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 
      Mie, Japan.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Mutagens)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/administration & dosage/*pharmacology
MH  - Administration, Intravenous
MH  - Animals
MH  - Body Weight/drug effects
MH  - Female
MH  - Fluorescent Dyes/administration & dosage/pharmacology
MH  - Lethal Dose 50
MH  - Male
MH  - Mice
MH  - Mutagens/administration & dosage/*pharmacology
MH  - *Toxicity Tests, Acute
OTO - NOTNLM
OT  - Acridine orange
OT  - LD50
OT  - acute toxicity
EDAT- 2014/07/02 06:00
MHDA- 2015/02/24 06:00
CRDT- 2014/07/02 06:00
PHST- 2014/07/02 06:00 [entrez]
PHST- 2014/07/02 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
AID - 28/4/523 [pii]
PST - ppublish
SO  - In Vivo. 2014 Jul-Aug;28(4):523-7.