PMID- 24982319
OWN - NLM
STAT- MEDLINE
DCOM- 20150220
LR  - 20220408
IS  - 1098-6618 (Electronic)
IS  - 0893-8512 (Print)
IS  - 0893-8512 (Linking)
VI  - 27
IP  - 3
DP  - 2014 Jul
TI  - Molecular and nonmolecular diagnostic methods for invasive fungal infections.
PG  - 490-526
LID - 10.1128/CMR.00091-13 [doi]
AB  - Invasive fungal infections constitute a serious threat to an ever-growing 
      population of immunocompromised individuals and other individuals at risk. 
      Traditional diagnostic methods, such as histopathology and culture, which are 
      still considered the gold standards, have low sensitivity, which underscores the 
      need for the development of new means of detecting fungal infectious agents. 
      Indeed, novel serologic and molecular techniques have been developed and are 
      currently under clinical evaluation. Tests like the galactomannan antigen test 
      for aspergillosis and the beta-glucan test for invasive Candida spp. and molds, as 
      well as other antigen and antibody tests, for Cryptococcus spp., Pneumocystis 
      spp., and dimorphic fungi, have already been established as important diagnostic 
      approaches and are implemented in routine clinical practice. On the other hand, 
      PCR and other molecular approaches, such as matrix-assisted laser desorption 
      ionization (MALDI) and fluorescence in situ hybridization (FISH), have proved 
      promising in clinical trials but still need to undergo standardization before 
      their clinical use can become widespread. The purpose of this review is to 
      highlight the different diagnostic approaches that are currently utilized or 
      under development for invasive fungal infections and to identify their 
      performance characteristics and the challenges associated with their use.
CI  - Copyright (c) 2014, American Society for Microbiology. All Rights Reserved.
FAU - Arvanitis, Marios
AU  - Arvanitis M
AD  - Division of Infectious Diseases, Warren Alpert Medical School of Brown 
      University, Providence, Rhode Island, USA.
FAU - Anagnostou, Theodora
AU  - Anagnostou T
AD  - Division of Infectious Diseases, Warren Alpert Medical School of Brown 
      University, Providence, Rhode Island, USA.
FAU - Fuchs, Beth Burgwyn
AU  - Fuchs BB
AD  - Division of Infectious Diseases, Warren Alpert Medical School of Brown 
      University, Providence, Rhode Island, USA.
FAU - Caliendo, Angela M
AU  - Caliendo AM
AD  - Division of Infectious Diseases, Warren Alpert Medical School of Brown 
      University, Providence, Rhode Island, USA Division of General Internal Medicine, 
      Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA.
FAU - Mylonakis, Eleftherios
AU  - Mylonakis E
AD  - Division of Infectious Diseases, Warren Alpert Medical School of Brown 
      University, Providence, Rhode Island, USA emylonakis@lifespan.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Clin Microbiol Rev
JT  - Clinical microbiology reviews
JID - 8807282
SB  - IM
MH  - Animals
MH  - Fungi/*physiology
MH  - Humans
MH  - Microbiological Techniques/*methods/standards
MH  - Mycoses/*diagnosis/*microbiology
MH  - Reproducibility of Results
PMC - PMC4135902
EDAT- 2014/07/02 06:00
MHDA- 2015/02/24 06:00
PMCR- 2015/07/01
CRDT- 2014/07/02 06:00
PHST- 2014/07/02 06:00 [entrez]
PHST- 2014/07/02 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - 27/3/490 [pii]
AID - 00091-13 [pii]
AID - 10.1128/CMR.00091-13 [doi]
PST - ppublish
SO  - Clin Microbiol Rev. 2014 Jul;27(3):490-526. doi: 10.1128/CMR.00091-13.