PMID- 24982554
OWN - NLM
STAT- MEDLINE
DCOM- 20150306
LR  - 20211021
IS  - 1928-9022 (Electronic)
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 78
IP  - 3
DP  - 2014 Jul
TI  - Reliability of manual measurements of corneal thickness obtained from healthy 
      canine eyes using spectral-domain optical coherence tomography (SD-OCT).
PG  - 221-5
AB  - The purpose of this study was to manually measure corneal thickness in canine 
      eyes using a spectral-domain optical coherence tomography (SD-OCT) device and to 
      assess intra- and inter-observer reliability of this technique. Twenty healthy 
      dogs with a mean age of 4.7 y were examined. A 6-mm corneal pachymetry protocol 
      was carried out by 1 operator using 1 SD-OCT device in both eyes of each animal. 
      Measurements were obtained manually and in duplicate by 2 independent 
      investigators (> 24 h apart), using the built-in caliper function. Measurements 
      included epithelial thickness (ET), non-epithelial thickness (NET), and central 
      corneal thickness (CCT). The overall mean ET, NET, and CCT for all eyes examined 
      were 72.3 +/- 4.6 mum, 538.9 +/- 42.5 mum, and 611.2 +/- 40.3 mum, respectively. There was 
      no significant difference in ET, NET, or CCT based on the eye examined [oculus 
      dexter (OD) versus oculus sinister (OS)], age, or gender of the animal. There was 
      no significant difference in replicate measurements of ET, NET, or CCT done by 
      the same operator, although a small but significant difference was noted between 
      operators for ET measurements only. The mean difference in ET between operators 
      was 0.6 mum (P = 0.03). The coefficient of variation ranged from 0.5% to 9.27% and 
      intraclass correlation coefficient ranged from 0.35 to 0.97. Based on these 
      results, manual measurements of corneal thickness in canine eyes using a portable 
      SD-OCT device provided ET, NET, and CCT measurements with clinically acceptable 
      intra- and inter-observer reliability.
FAU - Alario, Anthony F
AU  - Alario AF
AD  - Department of Clinical Sciences, Tufts Cummings School of Veterinary Medicine, 
      North Grafton, Massachusetts 01536, USA.
FAU - Pirie, Christopher G
AU  - Pirie CG
AD  - Department of Clinical Sciences, Tufts Cummings School of Veterinary Medicine, 
      North Grafton, Massachusetts 01536, USA.
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
SB  - IM
MH  - Animals
MH  - Cornea/*anatomy & histology
MH  - Dogs/*anatomy & histology
MH  - Female
MH  - Male
MH  - Observer Variation
MH  - Reproducibility of Results
MH  - Tomography, Optical Coherence/methods/statistics & numerical data/*veterinary
PMC - PMC4068414
EDAT- 2014/07/02 06:00
MHDA- 2015/03/07 06:00
PMCR- 2015/01/01
CRDT- 2014/07/02 06:00
PHST- 2013/04/18 00:00 [received]
PHST- 2013/08/19 00:00 [accepted]
PHST- 2014/07/02 06:00 [entrez]
PHST- 2014/07/02 06:00 [pubmed]
PHST- 2015/03/07 06:00 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - cjvr_07_221 [pii]
PST - ppublish
SO  - Can J Vet Res. 2014 Jul;78(3):221-5.