PMID- 24982682
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140701
LR  - 20211021
IS  - 1710-1484 (Print)
IS  - 1710-1492 (Electronic)
IS  - 1710-1484 (Linking)
VI  - 10
IP  - 1
DP  - 2014
TI  - Enhancement of OVA-induced murine lung eosinophilia by co-exposure to 
      contamination levels of LPS in Asian sand dust and heated dust.
PG  - 30
LID - 10.1186/1710-1492-10-30 [doi]
AB  - BACKGROUND: A previous study has shown that the aggravation of Asian sand dust 
      (ASD) on ovalbumin (OVA)-induced lung eosinphilia was more severe in 
      lipopolysaccharide (LPS)-rich ASD than in SiO2-rich ASD. Therefore, the effects 
      of different LPS contamination levels in ASD on the aggravation of OVA-induced 
      lung eosinophilia were investigated in the present study. METHODS: Before 
      beginning the in vivo experiment, we investigated whether the ultra-pure LPS 
      would act only on TLR4 or not using bone marrow-derived macrophages (BMDMs) of 
      wild-type, TLR2-/-, TLR4-/- and MyD88-/- BALB/c mice. ASD collected from the 
      desert was heated to remove toxic organic substances (H-ASD). BALB/c mice were 
      instilled intratracheally with 12 different testing samples prepared with LPS 
      (1 ng and 10 ng), H-ASD, and OVA in a normal saline solution. The lung pathology, 
      cytological profiles in the bronchoalveolar lavage fluid (BALF), the levels of 
      inflammatory cytokines/chemokines in BALF and OVA-specific immunoglobulin in 
      serum were investigated. RESULTS: The LPS exhibited no response to the production 
      of TNF-alpha and IL-6 in BMDMs from TLR4-/-, but did from TLR2-/-. H-ASD aggravated 
      the LPS-induced neutrophilic lung inflammation. In the presence of OVA, LPS 
      increased the level of eosinophils slightly and induced trace levels of Th2 
      cytokines IL-5 and IL-13 at the levels of 1 ng and 10 ng. In the presence of OVA 
      and H-ASD, LPS induced severe eosinophil infiltration and proliferation of goblet 
      cells in the airways as well as remarkable increases in Th2 cytokines IL-5 and 
      IL-13 in BALF. The mixture containing LPS (1 ng) showed adjuvant activity on 
      OVA-specific IgE and IgG1 production. CONCLUSIONS: The results suggest that H-ASD 
      with naturally-occurring levels of LPS enhances OVA-induced lung eosinophilia via 
      increases in Th2-mediated cytokines and antigen-specific immunoglobulin. These 
      results indicate that LPS is a strong candidate for being a major aggravating 
      substance in ASD.
FAU - Ren, Yahao
AU  - Ren Y
AD  - Department of Nutritional and Food Hygiene, College of Public Health, China 
      Medical University, Shenyang, China.
FAU - Ichinose, Takamichi
AU  - Ichinose T
AD  - Department of Health Sciences, Oita University of Nursing and Health Sciences, 
      Oita 870-1201, Japan.
FAU - He, Miao
AU  - He M
AD  - Department of Environmental and Occupational Health, College of Public Health, 
      China Medical University, Shenyang, China.
FAU - Song, Yuan
AU  - Song Y
AD  - Department of Immunology and Parasitology, School of Medicine, University of 
      Occupational and Environmental Health, Japan, Fukuoka 807-8555, Japan.
FAU - Yoshida, Yasuhiro
AU  - Yoshida Y
AD  - Department of Immunology and Parasitology, School of Medicine, University of 
      Occupational and Environmental Health, Japan, Fukuoka 807-8555, Japan.
FAU - Yoshida, Seiichi
AU  - Yoshida S
AD  - Department of Health Sciences, Oita University of Nursing and Health Sciences, 
      Oita 870-1201, Japan.
FAU - Nishikawa, Masataka
AU  - Nishikawa M
AD  - Environmental Chemistry Division, National Institute for Environmental Studies, 
      Ibaraki 305-8506, Japan.
FAU - Takano, Hirohisa
AU  - Takano H
AD  - Environmental Health Division, Department of Environmental Engineering, Graduate 
      School of Engineering, Kyoto University, Kyoto 615-8530, Japan.
FAU - Sun, Guifan
AU  - Sun G
AD  - Department of Environmental and Occupational Health, College of Public Health, 
      China Medical University, Shenyang, China.
FAU - Shibamoto, Takayuki
AU  - Shibamoto T
AD  - Department of Environmental Toxicology, University of California, Davis, CA 
      95616, USA.
LA  - eng
PT  - Journal Article
DEP - 20140609
PL  - England
TA  - Allergy Asthma Clin Immunol
JT  - Allergy, asthma, and clinical immunology : official journal of the Canadian 
      Society of Allergy and Clinical Immunology
JID - 101244313
PMC - PMC4058696
OTO - NOTNLM
OT  - Asian sand dust
OT  - Asthma
OT  - Cytokine and chemokine
OT  - Lipopolysaccharide
OT  - Lung eosinophilia
OT  - Ovalbumin
EDAT- 2014/07/02 06:00
MHDA- 2014/07/02 06:01
PMCR- 2014/06/09
CRDT- 2014/07/02 06:00
PHST- 2014/03/13 00:00 [received]
PHST- 2014/05/03 00:00 [accepted]
PHST- 2014/07/02 06:00 [entrez]
PHST- 2014/07/02 06:00 [pubmed]
PHST- 2014/07/02 06:01 [medline]
PHST- 2014/06/09 00:00 [pmc-release]
AID - 1710-1492-10-30 [pii]
AID - 10.1186/1710-1492-10-30 [doi]
PST - epublish
SO  - Allergy Asthma Clin Immunol. 2014 Jun 9;10(1):30. doi: 10.1186/1710-1492-10-30. 
      eCollection 2014.