PMID- 24984919
OWN - NLM
STAT- MEDLINE
DCOM- 20150417
LR  - 20140806
IS  - 1618-0402 (Electronic)
IS  - 0940-9602 (Linking)
VI  - 196
IP  - 5
DP  - 2014 Sep
TI  - BDNF and its TrkB receptor in human fracture healing.
PG  - 286-95
LID - S0940-9602(14)00120-4 [pii]
LID - 10.1016/j.aanat.2014.06.001 [doi]
AB  - Fracture healing is a physiological process of repair which proceeds in stages, 
      each characterized by a different predominant tissue in the fracture gap. Matrix 
      reorganization is regulated by cytokines and growth factors. Neurotrophins and 
      their receptors might be of importance to osteoblasts and endothelial cells 
      during fracture healing. The aim of this study was to examine the presence of 
      brain-derived neurotrophic factor (BDNF) and its tropomyosin-related kinase B 
      receptor (TrkB) during human fracture healing. BDNF and TrkB were investigated in 
      samples from human fracture gaps and cultured cells using RT-PCR, Western blot, 
      and immunohistochemistry. Endothelial cells and osteoblastic cell lines 
      demonstrated a cytoplasmic staining pattern of BDNF and TrkB in vitro. At the 
      mRNA level, BDNF and TrkB were expressed in the initial and osteoid formation 
      phase of human fracture healing. In the granulation tissue of fracture gap, both 
      proteins--BDNF and TrkB--are concentrated in endothelial and osteoblastic cells 
      at the margins of woven bone suggesting their involvement in the formation of new 
      vessels. There was no evidence of BDNF or TrkB during fracture healing in 
      chondrocytes of human enchondral tissue. Furthermore, BDNF is absent in mature 
      bone. Taken together, BDNF and TrkB are involved in vessel formation and 
      osteogenic processes during human fracture healing. The detection of BDNF and its 
      TrkB receptor during various stages of the bone formation process in human 
      fracture gap tissue were shown for the first time. The current study reveals that 
      both proteins are up-regulated in human osteoblasts and endothelial cells in 
      fracture healing.
CI  - Copyright (c) 2014 Elsevier GmbH. All rights reserved.
FAU - Kilian, Olaf
AU  - Kilian O
AD  - Laboratory of Experimental Trauma Surgery, University of Giessen, Kerkraderstr. 
      9, 35392 Giessen, Germany; Department of Orthopedics and Trauma, Zentralklinik 
      Bad Berka, Robert-Koch-Allee 9, 99437 Bad Berka, Germany. Electronic address: 
      olaf.kilian@gmx.de.
FAU - Hartmann, Sonja
AU  - Hartmann S
AD  - Laboratory of Experimental Trauma Surgery, University of Giessen, Kerkraderstr. 
      9, 35392 Giessen, Germany. Electronic address: 
      Sonja.hartmann@chiru.med.uni-giessen.de.
FAU - Dongowski, Nicole
AU  - Dongowski N
AD  - Department of Trauma Surgery, Asklepios Klinik Lich, Goethestr. 4, 35423 Lich, 
      Germany. Electronic address: Nicole.dongowski@chiru.med.uni-giessen.de.
FAU - Karnati, Srikanth
AU  - Karnati S
AD  - Institute for Anatomy and Cell Biology II, Division of Medical Cell Biology, 
      University of Giessen, Aulweg 123, 35392 Giessen, Germany. Electronic address: 
      Srikanth.karnati@anatomie.med.uni-giessen.de.
FAU - Baumgart-Vogt, Eveline
AU  - Baumgart-Vogt E
AD  - Institute for Anatomy and Cell Biology II, Division of Medical Cell Biology, 
      University of Giessen, Aulweg 123, 35392 Giessen, Germany. Electronic address: 
      Eveline.baumgart-vogt@anatomie.med.uni-giessen.de.
FAU - Hartel, Frauke V
AU  - Hartel FV
AD  - Institute of Physiology, University of Giessen, Aulweg 129, 35392 Giessen, 
      Germany. Electronic address: Frauke.v.haertel@physiologie.med.uni-giesssen.de.
FAU - Noll, Thomas
AU  - Noll T
AD  - Institute of Physiology, University of Giessen, Aulweg 129, 35392 Giessen, 
      Germany. Electronic address: Thomas.noll@physiologie.med.uni-giessen.de.
FAU - Schnettler, Reinhard
AU  - Schnettler R
AD  - Laboratory of Experimental Trauma Surgery, University of Giessen, Kerkraderstr. 
      9, 35392 Giessen, Germany; Department of Trauma Surgery, University of Giessen, 
      Rudolf-Buchheim-Str. 7, 35385 Giessen, Germany. Electronic address: 
      Reinhard.schnettler@chiru.med.uni-giessen.de.
FAU - Lips, Katrin Susanne
AU  - Lips KS
AD  - Laboratory of Experimental Trauma Surgery, University of Giessen, Kerkraderstr. 
      9, 35392 Giessen, Germany. Electronic address: 
      Katrin.s.lips@chiru.med.uni-giessen.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140616
PL  - Germany
TA  - Ann Anat
JT  - Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische 
      Gesellschaft
JID - 100963897
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Development/physiology
MH  - Bone and Bones/metabolism/*physiology
MH  - Brain-Derived Neurotrophic Factor/metabolism/*physiology
MH  - Cell Line
MH  - Female
MH  - Fracture Healing/*physiology
MH  - Humans
MH  - Ilium/physiology
MH  - Male
MH  - Middle Aged
MH  - Osteoblasts/physiology
MH  - Receptor, trkB/metabolism/*physiology
MH  - Young Adult
OTO - NOTNLM
OT  - Angiogenesis
OT  - Blood vessel
OT  - Bone
OT  - Brain-derived neurotrophic factor
OT  - Neurotrophin
OT  - Osteoblast
OT  - Tropomyosin-related kinases
EDAT- 2014/07/06 06:00
MHDA- 2015/04/18 06:00
CRDT- 2014/07/03 06:00
PHST- 2014/02/07 00:00 [received]
PHST- 2014/06/05 00:00 [revised]
PHST- 2014/06/06 00:00 [accepted]
PHST- 2014/07/03 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/04/18 06:00 [medline]
AID - S0940-9602(14)00120-4 [pii]
AID - 10.1016/j.aanat.2014.06.001 [doi]
PST - ppublish
SO  - Ann Anat. 2014 Sep;196(5):286-95. doi: 10.1016/j.aanat.2014.06.001. Epub 2014 Jun 
      16.