PMID- 24985006
OWN - NLM
STAT- MEDLINE
DCOM- 20150209
LR  - 20211203
IS  - 1873-1244 (Electronic)
IS  - 0899-9007 (Linking)
VI  - 30
IP  - 7-8
DP  - 2014 Jul-Aug
TI  - Whey protein modifies gene expression related to protein metabolism affecting 
      muscle weight in resistance-exercised rats.
PG  - 876-81
LID - S0899-9007(13)00558-3 [pii]
LID - 10.1016/j.nut.2013.12.007 [doi]
AB  - OBJECTIVE: The aim of this study was to evaluate the effects of resistance 
      exercise on the mRNA expression of muscle mammalian target of rapamycin (mTOR), 
      muscle-specific RING finger-1 (MuRF-1), and muscle atrophy F-box (MAFbx) in the 
      presence or absence of whey protein ingestion. We hypothesized that resistance 
      exercise in combination with whey protein ingestion alters the gene expression of 
      proteins related to muscle protein synthesis (mTOR) and/or degradation (MuRF-1 
      and MAFbx), thus affecting muscle weight gain in rats. METHODS: Thirty-two male 
      Fischer rats were randomly assigned to the following four experimental groups (n 
      = 8/group): Control sedentary, control exercised, whey protein sedentary, and 
      whey protein exercised. Exercise consisted of inducing the animals to perform 
      sets of jumps for 8 wk. Body weight gain, muscle weights, food intake, and 
      feeding efficiency were evaluated. Gene expressions were analyzed by quantitative 
      real-time reverse transcription polymerase chain reaction. Statistical evaluation 
      was performed using a two-way analysis of variance with a Tukey post hoc test. 
      RESULTS: Whey protein exercised rats exhibited higher body and muscle weight gain 
      compared with control-exercised rats (P = 0.032). The expression of mTOR was 
      reduced by exercise but increased when whey protein was consumed as a dietary 
      protein (P = 0.005). MuRF-1 expression was reduced by exercise (P < 0.001), 
      whereas MAFbx was reduced only by whey protein ingestion (P = 0.008) independent 
      of exercise. CONCLUSIONS: A reduction in MAFbx gene transcription induced by whey 
      protein and the interaction between exercise and whey protein ingestion on mTOR 
      gene expression contributed significantly to differences in body and muscle 
      weight gain.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Haraguchi, Fabiano Kenji
AU  - Haraguchi FK
AD  - Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, 
      Ouro Preto, Minas Gerais, Brazil; Department of Integrated Health (DEIS), Federal 
      University of Espirito Santo, Vitoria, Espirito Santo, Brazil. Electronic 
      address: fabianokenji@gmail.com.
FAU - de Brito Magalhaes, Cintia Lopes
AU  - de Brito Magalhaes CL
AD  - Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, 
      Ouro Preto, Minas Gerais, Brazil; Department of Biological Sciences (DECBI), 
      Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
FAU - Neves, Leandro Xavier
AU  - Neves LX
AD  - Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, 
      Ouro Preto, Minas Gerais, Brazil.
FAU - dos Santos, Rinaldo Cardoso
AU  - dos Santos RC
AD  - Department of Foods, School of Nutrition, Federal University of Ouro Preto, Ouro 
      Preto, Minas Gerais, Brazil.
FAU - Pedrosa, Maria Lucia
AU  - Pedrosa ML
AD  - Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, 
      Ouro Preto, Minas Gerais, Brazil; Department of Biological Sciences (DECBI), 
      Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
FAU - Silva, Marcelo Eustaquio
AU  - Silva ME
AD  - Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, 
      Ouro Preto, Minas Gerais, Brazil; Department of Foods, School of Nutrition, 
      Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131214
PL  - United States
TA  - Nutrition
JT  - Nutrition (Burbank, Los Angeles County, Calif.)
JID - 8802712
RN  - 0 (Dietary Proteins)
RN  - 0 (Milk Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tripartite Motif Proteins)
RN  - 0 (Whey Proteins)
RN  - EC 2.3.2.27 (Trim63 protein, rat)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Body Weight/drug effects
MH  - Dietary Proteins/*pharmacology
MH  - Gene Expression/drug effects
MH  - Male
MH  - Milk Proteins/*pharmacology
MH  - Movement
MH  - Muscle Proteins/genetics/*metabolism
MH  - Muscle, Skeletal/*drug effects/metabolism
MH  - Organ Size/drug effects
MH  - *Physical Conditioning, Animal
MH  - Plyometric Exercise
MH  - Protein Biosynthesis
MH  - Proteolysis
MH  - RNA, Messenger/metabolism
MH  - Random Allocation
MH  - Rats, Inbred F344
MH  - *Resistance Training
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
MH  - Tripartite Motif Proteins
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
MH  - Whey Proteins
OTO - NOTNLM
OT  - Dietary protein
OT  - Exercise
OT  - MAFbx
OT  - MuRF-1
OT  - Protein degradation
OT  - Protein synthesis
OT  - mTOR
EDAT- 2014/07/06 06:00
MHDA- 2015/02/11 06:00
CRDT- 2014/07/03 06:00
PHST- 2013/08/28 00:00 [received]
PHST- 2013/11/06 00:00 [revised]
PHST- 2013/12/05 00:00 [accepted]
PHST- 2014/07/03 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
AID - S0899-9007(13)00558-3 [pii]
AID - 10.1016/j.nut.2013.12.007 [doi]
PST - ppublish
SO  - Nutrition. 2014 Jul-Aug;30(7-8):876-81. doi: 10.1016/j.nut.2013.12.007. Epub 2013 
      Dec 14.