PMID- 24985010
OWN - NLM
STAT- MEDLINE
DCOM- 20150209
LR  - 20181202
IS  - 1873-1244 (Electronic)
IS  - 0899-9007 (Linking)
VI  - 30
IP  - 7-8
DP  - 2014 Jul-Aug
TI  - Hypersensitivity linked to exposure of broad bean protein(s) in allergic patients 
      and BALB/c mice.
PG  - 903-14
LID - S0899-9007(13)00563-7 [pii]
LID - 10.1016/j.nut.2013.11.024 [doi]
AB  - OBJECTIVE: Broad bean (Vicia faba L.), a common vegetable, belongs to the family 
      Fabaceae and is consumed worldwide. Limited studies have been done on 
      allergenicity of broad beans. The aim of this study was to determine if broad 
      bean proteins have the ability to elicit allergic responses due to the presence 
      of clinically relevant allergenic proteins. METHODS: Simulated gastric fluid 
      (SGF) assay and immunoglobulin E (IgE) immunoblotting were carried out to 
      identify pepsin-resistant and IgE-binding proteins. The allergenicity of broad 
      beans was assessed in allergic patients, BALB/c mice, splenocytes, and RBL-2H3 
      cells. RESULTS: Eight broad bean proteins of approximate molecular weight 70, 60, 
      48, 32, 23, 19, 15, and 10 kDa that remained undigested in SGF, showed 
      IgE-binding capacity as well. Of 127 allergic patients studied, broad bean 
      allergy was evident in 16 (12%). Mice sensitized with broad bean showed increased 
      levels of histamine, total and specific IgE, and severe signs of systemic 
      anaphylaxis compared with controls. Enhanced levels of histamine, prostaglandin 
      D2, cysteinyl leukotriene, and beta-hexosaminidase release were observed in the 
      primed RBL-2H3 cells following broad bean exposure. The levels of interleukin 
      IL-4, IL-5, IL-13 and regulated on activation, normal T-cell expressed and 
      secreted were found enhanced in broad bean-treated splenocytes culture 
      supernatant compared with controls. CONCLUSION: This study inferred that broad 
      bean proteins have the ability to elicit allergic responses due to the presence 
      of clinically relevant allergenic proteins.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Kumar, Dinesh
AU  - Kumar D
AD  - Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology 
      Research, Lucknow, India; Department of Biotechnology, Jamia Hamdard, Hamdard 
      Nagar, New Delhi, India.
FAU - Kumar, Sandeep
AU  - Kumar S
AD  - Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology 
      Research, Lucknow, India.
FAU - Verma, Alok K
AU  - Verma AK
AD  - Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology 
      Research, Lucknow, India.
FAU - Sharma, Akanksha
AU  - Sharma A
AD  - Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology 
      Research, Lucknow, India.
FAU - Tripathi, Anurag
AU  - Tripathi A
AD  - Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology 
      Research, Lucknow, India.
FAU - Chaudhari, Bhushan P
AU  - Chaudhari BP
AD  - Central Pathology Lab, Gehru Campus, CSIR-Indian Institute of Toxicology 
      Research, Lucknow, India.
FAU - Kant, Surya
AU  - Kant S
AD  - Department of Pulmonary Medicine, King George Medical University, Lucknow, India.
FAU - Das, Mukul
AU  - Das M
AD  - Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology 
      Research, Lucknow, India.
FAU - Jain, Swatantra K
AU  - Jain SK
AD  - Department of Biotechnology, Jamia Hamdard, Hamdard Nagar, New Delhi, India.
FAU - Dwivedi, Premendra D
AU  - Dwivedi PD
AD  - Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology 
      Research, Lucknow, India. Electronic address: pddwivedi@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131216
PL  - United States
TA  - Nutrition
JT  - Nutrition (Burbank, Los Angeles County, Calif.)
JID - 8802712
RN  - 0 (Dietary Proteins)
RN  - 0 (Interleukins)
RN  - 0 (Leukotrienes)
RN  - 0 (Plant Proteins)
RN  - 0 (cysteinyl-leukotriene)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - K848JZ4886 (Cysteine)
RN  - RXY07S6CZ2 (Prostaglandin D2)
SB  - IM
MH  - Anaphylaxis
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cysteine/metabolism
MH  - *Diet
MH  - Dietary Proteins/*immunology/metabolism
MH  - Female
MH  - *Food Hypersensitivity/metabolism
MH  - Gastric Juice/metabolism
MH  - Histamine/*metabolism
MH  - Humans
MH  - Hypersensitivity/metabolism
MH  - Immunoglobulin E/*metabolism
MH  - Interleukins/metabolism
MH  - Leukotrienes/metabolism
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Molecular Weight
MH  - Plant Proteins/*immunology/metabolism
MH  - Prostaglandin D2/metabolism
MH  - Seeds/chemistry/immunology
MH  - Spleen/drug effects
MH  - T-Lymphocytes/metabolism
MH  - Vicia faba/chemistry/*immunology
MH  - beta-N-Acetylhexosaminidases/metabolism
OTO - NOTNLM
OT  - Allergens
OT  - Anaphylaxis
OT  - Broad bean
OT  - Cytokines
OT  - Food allergy
EDAT- 2014/07/06 06:00
MHDA- 2015/02/11 06:00
CRDT- 2014/07/03 06:00
PHST- 2013/08/05 00:00 [received]
PHST- 2013/11/13 00:00 [revised]
PHST- 2013/11/25 00:00 [accepted]
PHST- 2014/07/03 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
AID - S0899-9007(13)00563-7 [pii]
AID - 10.1016/j.nut.2013.11.024 [doi]
PST - ppublish
SO  - Nutrition. 2014 Jul-Aug;30(7-8):903-14. doi: 10.1016/j.nut.2013.11.024. Epub 2013 
      Dec 16.