PMID- 24985206
OWN - NLM
STAT- MEDLINE
DCOM- 20150209
LR  - 20161125
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 9
IP  - 2
DP  - 2014 Jun
TI  - Arginine-glycine-glutamine and serine-isoleucine-lysine-valine-alanine-valine 
      modified poly(l-lactide) films: bioactive molecules used for surface grafting to 
      guide cellular contractile phenotype.
PG  - 029002
LID - 10.1116/1.4864432 [doi]
AB  - Tissue engineering is defined as "an interdisciplinary field that applies the 
      principles of engineering and life sciences toward the development of biological 
      substitutes that restore, maintain, or improve tissue function." The biological 
      substitutes can be developed with the help of natural or synthetic materials. 
      Polymeric materials are primarily used, because of the high variability in 
      mechanical, physical, and chemical properties. Biodegradable polymers are object 
      of the majority of studies, because of the ability to be degraded by the host 
      organism, avoiding late stent thrombosis unlike permanent grafts. Poly-l-lactide 
      acid (PLLA) is one of the most used polymers in research. In order to improve the 
      material's bioactivity, in this work, PLLA surface was modified by grafted 
      arginine-glycine-glutamine (RGD), a fibronectin derived adhesion motif, and 
      serine-isoleucine-lysine-valine-alanine-valine (SIKVAV), a laminin derived motif, 
      and rat cardiac (H9C2) and mouse (C2C12) myoblasts proliferation and 
      differentiation on modified PLLA were evaluated. In order to verify the surface 
      modification, x-ray photoelectron spectroscopy analysis was performed. After 
      seeding, cells' viability was confirmed by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay while 
      proliferating cell nuclear antigen expression was used to investigate cell 
      proliferation. Myf5, Myogenin and Myosin heavy chain were used to analyze cell 
      differentiation. Moreover, RGD peptide slightly inhibited rat myoblast (H9C2) 
      proliferation, whereas less strong effect was observed on C2C12. However, both 
      cell lines showed to enhance the contractile phenotype in the presence of SIKVAV 
      peptides. These results suggest that bioactive molecules grafting could be useful 
      on polymeric scaffolds for guiding cell phenotype expression, and, to ultimately 
      maintain adequate biological characteristics suitable for the tissue functional 
      regeneration.
FAU - Boccafoschi, Francesca
AU  - Boccafoschi F
AD  - Human Anatomy Laboratory, Department of Health Sciences, University of Piemonte 
      Orientale "A. Avogadro", 28100 Novara, Italy.
FAU - Fusaro, Luca
AU  - Fusaro L
AD  - Human Anatomy Laboratory, Department of Health Sciences, University of Piemonte 
      Orientale "A. Avogadro", 28100 Novara, Italy.
FAU - Botta, Margherita
AU  - Botta M
AD  - Human Anatomy Laboratory, Department of Health Sciences, University of Piemonte 
      Orientale "A. Avogadro", 28100 Novara, Italy.
FAU - Ramella, Martina
AU  - Ramella M
AD  - Human Anatomy Laboratory, Department of Health Sciences, University of Piemonte 
      Orientale "A. Avogadro", 28100 Novara, Italy.
FAU - Chevallier, Pascale
AU  - Chevallier P
AD  - Laboratory for Biomaterials and Bioengineering, Department of Min-Met-Materials 
      Engineering, Laval University and University Hospital Research Center, Quebec 
      City, G1V 0A6, Canada.
FAU - Mantovani, Diego
AU  - Mantovani D
AD  - Laboratory for Biomaterials and Bioengineering, Department of Min-Met-Materials 
      Engineering, Laval University and University Hospital Research Center, Quebec 
      City, G1V 0A6, Canada.
FAU - Cannas, Mario
AU  - Cannas M
AD  - Human Anatomy Laboratory, Department of Health Sciences, University of Piemonte 
      Orientale "A. Avogadro", 28100 Novara, Italy.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 0 (Biocompatible Materials)
RN  - 0 (Fibronectins)
RN  - 0 (Myogenic Regulatory Factor 5)
RN  - 0 (Oligopeptides)
RN  - 0 (Polyesters)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
SB  - IM
MH  - Amino Acid Motifs
MH  - Animals
MH  - Biocompatible Materials/chemistry/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Fibronectins/*chemistry
MH  - Mice
MH  - Myogenic Regulatory Factor 5/metabolism
MH  - Oligopeptides/*chemistry
MH  - Phenotype
MH  - Photoelectron Spectroscopy
MH  - Polyesters/*chemistry/pharmacology
MH  - Rats
MH  - Surface Properties
MH  - *Tissue Engineering
EDAT- 2014/07/06 06:00
MHDA- 2015/02/11 06:00
CRDT- 2014/07/03 06:00
PHST- 2014/07/03 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
AID - 10.1116/1.4864432 [doi]
PST - ppublish
SO  - Biointerphases. 2014 Jun;9(2):029002. doi: 10.1116/1.4864432.