PMID- 24986468
OWN - NLM
STAT- MEDLINE
DCOM- 20141126
LR  - 20211021
IS  - 1476-511X (Electronic)
IS  - 1476-511X (Linking)
VI  - 13
DP  - 2014 Jul 1
TI  - Immunohistochemical profiling of the heat shock response in obese non-diabetic 
      subjects revealed impaired expression of heat shock proteins in the adipose 
      tissue.
PG  - 106
LID - 10.1186/1476-511X-13-106 [doi]
AB  - BACKGROUND: Obesity is characterized by a chronic low-grade inflammation and 
      altered stress responses in key metabolic tissues. Impairment of heat shock 
      response (HSR) has been already linked to diabetes and insulin resistance as 
      reflected by decrease in heat shock proteins (HSPs) expression. However, the 
      status of HSR in non-diabetic human obese has not yet been elucidated. The aim of 
      the current study was to investigate whether obesity triggers a change in the HSR 
      pattern and the impact of physical exercise on this pattern at protein and mRNA 
      levels. METHODS: Two groups of adult non-diabetic human subjects consisting of 
      lean and obese (n = 47 for each group) were enrolled in this study. The 
      expression pattern of HSP-27, DNAJB3/HSP-40, HSP-60, HSC-70, HSP72, HSP-90 and 
      GRP-94 in the adipose tissue was primarily investigated by immunohistochemistry 
      and then complemented by western blot and qRT-PCR in Peripheral blood mononuclear 
      cells (PBMCs). HSPs expression levels were correlated with various physical, 
      clinical and biochemical parameters. We have also explored the effect of a 
      3-month moderate physical exercise on the HSPs expression pattern in obese 
      subjects. RESULTS: Obese subjects displayed increased expression of HSP-60, 
      HSC-70, HSP-72, HSP-90 and GRP-94 and lower expression of DNAJB3/HSP-40 
      (P < 0.05). No differential expression was observed for HSP-27 between the two 
      groups. Higher levels of HSP-72 and GRP-94 proteins correlated positively with 
      the indices of obesity (body mass index and percent body fat) and circulating 
      levels of IFN-gamma-inducible protein 10 (IP-10) and RANTES chemokines. This 
      expression pattern was concomitant with increased inflammatory response in the 
      adipose tissue as monitored by increased levels of Interleukin-6 (IL-6), Tumor 
      necrosis factor-alpha (TNF-alpha), and RANTES (P < 0.05). Physical exercise reduced the 
      expression of various HSPs in obese to normal levels observed in lean subjects 
      with a parallel decrease in the endogenous levels of IL-6, TNF-alpha, and RANTES. 
      CONCLUSION: Taken together, these data indicate that obesity triggers 
      differential regulation of various components of the HSR in non-diabetic subjects 
      and a 3-month physical moderate exercise was sufficient to restore the normal 
      expression of HSPs in the adipose tissue with concomitant attenuation in the 
      inflammatory response.
FAU - Tiss, Ali
AU  - Tiss A
FAU - Khadir, Abdelkrim
AU  - Khadir A
FAU - Abubaker, Jehad
AU  - Abubaker J
FAU - Abu-Farha, Mohamed
AU  - Abu-Farha M
FAU - Al-Khairi, Irina
AU  - Al-Khairi I
FAU - Cherian, Preethi
AU  - Cherian P
FAU - John, Jeena
AU  - John J
FAU - Kavalakatt, Sina
AU  - Kavalakatt S
FAU - Warsame, Samia
AU  - Warsame S
FAU - Al-Ghimlas, Fahad
AU  - Al-Ghimlas F
FAU - Elkum, Naser
AU  - Elkum N
FAU - Behbehani, Kazem
AU  - Behbehani K
FAU - Dermime, Said
AU  - Dermime S
FAU - Dehbi, Mohammed
AU  - Dehbi M
AD  - Diabetes Research Centre, Qatar Biomedical Research Institute, Box: 5825, Doha, 
      Qatar. mdehbi@qf.org.qa.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140701
PL  - England
TA  - Lipids Health Dis
JT  - Lipids in health and disease
JID - 101147696
RN  - 0 (HSP72 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adipose Tissue/*metabolism
MH  - HSP72 Heat-Shock Proteins/metabolism
MH  - Heat-Shock Proteins/*metabolism
MH  - Heat-Shock Response/*physiology
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - Interleukin-6/blood
MH  - Obesity/blood/*metabolism
MH  - Tumor Necrosis Factor-alpha/blood
PMC - PMC4085713
EDAT- 2014/07/06 06:00
MHDA- 2014/12/15 06:00
PMCR- 2014/07/01
CRDT- 2014/07/03 06:00
PHST- 2014/04/26 00:00 [received]
PHST- 2014/06/17 00:00 [accepted]
PHST- 2014/07/03 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - 1476-511X-13-106 [pii]
AID - 10.1186/1476-511X-13-106 [doi]
PST - epublish
SO  - Lipids Health Dis. 2014 Jul 1;13:106. doi: 10.1186/1476-511X-13-106.