PMID- 24989005
OWN - NLM
STAT- MEDLINE
DCOM- 20150221
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 37
IP  - 7
DP  - 2014
TI  - St. John's wort promotes adipocyte differentiation and modulates NF-kappaB activation 
      in 3T3-L1 cells.
PG  - 1132-8
AB  - St. John's wort (SJW), or Hypericum perforatum, is a perennial herb that has been 
      used in the treatment of depression in several countries. Though its therapeutic 
      effect on depression has been extensively studied, its influence on metabolic 
      syndrome is yet to be fully characterized. Therefore, we investigated the effect 
      of SJW extract on adipocyte differentiation and its anti-inflammatory effects by 
      using 3T3-L1 preadipocytes. Oil Red O staining indicated that SJW promotes 
      adipocyte differentiation, while immunoblots indicated that SJW increases the 
      expression of peroxisome proliferator activated receptor gamma (PPARgamma), a nuclear 
      receptor regulating adipocyte differentiation, and adiponectin, an 
      anti-inflammatory adipokine. Furthermore, the anti-inflammatory activity of SJW 
      was demonstrated by its inhibition of the activation of nuclear factor-kappaB 
      (NF-kappaB), an inflammatory transcription factor. Stimulation of mature 3T3-L1 
      adipocytes by tumor necrosis factor-alpha (TNF-alpha) decreased the expression of the 
      NF-kappaB inhibitor IkappaBalpha, and increased its phosphorylation. Treatment with SJW 
      further decreased the TNF-alpha-induced perturbation in IkappaBalpha expression and 
      phosphorylation, which indicated that SJW mediated the inhibition of NF-kappaB 
      activation. In addition, SJW decreased the TNF-alpha-induced increase in the mRNA 
      levels of pro-inflammatory adipokines, interleukin-6 (IL-6), and monocyte 
      chemoattractant protein-1 (MCP-1). Collectively, our results indicate that SJW 
      treatment could promote adipocyte differentiation probably through its 
      anti-inflammatory activity, which in turn suggests that SJW has the potential to 
      minimize the risk factors of metabolic syndrome.
FAU - Hatano, Tomoko
AU  - Hatano T
AD  - School of Pharmaceutical Sciences, Mukogawa Women's University.
FAU - Sameshima, Yuka
AU  - Sameshima Y
FAU - Kawabata, Mami
AU  - Kawabata M
FAU - Yamada, Shizuo
AU  - Yamada S
FAU - Shinozuka, Kazumasa
AU  - Shinozuka K
FAU - Nakabayashi, Toshikatsu
AU  - Nakabayashi T
FAU - Mizuno, Hideya
AU  - Mizuno H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Adiponectin)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (NF-kappa B)
RN  - 0 (PPAR gamma)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/*drug effects/immunology/metabolism
MH  - Adiponectin/biosynthesis
MH  - Animals
MH  - Anti-Inflammatory Agents/isolation & purification/*pharmacology
MH  - Blotting, Western
MH  - Cell Culture Techniques
MH  - Cell Differentiation/*drug effects
MH  - Cell Survival/drug effects
MH  - Flowers/chemistry
MH  - Hypericum/*chemistry
MH  - Metabolic Syndrome/prevention & control
MH  - Mice
MH  - NF-kappa B/*antagonists & inhibitors
MH  - PPAR gamma/biosynthesis
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Real-Time Polymerase Chain Reaction
EDAT- 2014/07/06 06:00
MHDA- 2015/02/24 06:00
CRDT- 2014/07/04 06:00
PHST- 2014/07/04 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
AID - DN/JST.JSTAGE/bpb/b13-00989 [pii]
AID - 10.1248/bpb.b13-00989 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2014;37(7):1132-8. doi: 10.1248/bpb.b13-00989.