PMID- 24989426
OWN - NLM
STAT- MEDLINE
DCOM- 20150910
LR  - 20141224
IS  - 1440-1746 (Electronic)
IS  - 0815-9319 (Linking)
VI  - 30
IP  - 1
DP  - 2015 Jan
TI  - Lipopolysaccharide enhanced renal vascular response to endothelin-1 through ETA 
      overexpression in portal hypertensive rats.
PG  - 199-207
LID - 10.1111/jgh.12670 [doi]
AB  - BACKGROUND AND AIM: Hypo-perfusion resulting from intense renal vasoconstriction 
      is traditionally contributed to renal dysfunction in advanced liver disease, 
      although cumulative studies demonstrated renal vasodilatation with impaired 
      vascular contractility to endogenous vasoconstrictors in portal hypertension and 
      compensated liver cirrhosis. The pathophysiology of altered renal hemodynamics 
      remains unclear. This study, using a rat model of portal hypertension with 
      superimposed endotoxemia, was designed to delineate the evolution of renal 
      vascular reactivity and vaso-regulatory gene expression during liver disease 
      progression. METHODS: Rats were randomized into sham surgery (SHAM) or partial 
      portal vein ligation (PVL). Endotoxemia was induced by intraperitoneal injection 
      of lipopolysaccharide (LPS) on the seventh day following surgery. Isolated kidney 
      perfusion was performed at 0.5 h or 5 h after LPS to evaluate renal vascular 
      response to endothelin-1. RESULTS: In contrast to impaired vascular contractility 
      of SHAM rats, PVL rats displayed enhanced renal vascular reactivity to 
      endothelin-1 at 5 h following endotoxemia. There were extensive upregulations of 
      inducible nitric oxide synthase in kidney tissues of endotoxemic rats. The 
      changes of renal endothelin receptor type A (ETA ) level paralleled with the 
      changes of renal vascular reactivity in LPS-treated rats. Compared with SHAM 
      rats, PVL rats showed increased renal ETA and phosphorylated 
      extracellular-signal-regulated kinases 1/2 (p-ERK1/2) at 5 h after LPS. 
      CONCLUSION: LPS-induced systemic hypotension induces a paradoxical change of 
      renal vascular response to endothelin-1 between SHAM and PVL rats. LPS-induced 
      renal vascular hyperreactivity in PVL rats was associated with upregulation of 
      renal ETA and subsequent activation of ERK1/2 signaling.
CI  - (c) 2014 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing 
      Asia Pty Ltd.
FAU - Chuang, Chiao-Lin
AU  - Chuang CL
AD  - Division of General Medicine, Department of Medicine, Taipei Veterans General 
      Hospital, Taipei, Taiwan; Department of Medicine, National Yang-Ming University 
      School of Medicine, Taipei, Taiwan; Institute of Clinical Medicine, National 
      Yang-Ming University School of Medicine, Taipei, Taiwan.
FAU - Huang, Hui-Chun
AU  - Huang HC
FAU - Chang, Ching-Chih
AU  - Chang CC
FAU - Lee, Fa-Yauh
AU  - Lee FY
FAU - Wu, Jaw-Ching
AU  - Wu JC
FAU - Lee, Jing-Yi
AU  - Lee JY
FAU - Hsieh, Hsian-Guey
AU  - Hsieh HG
FAU - Lee, Shou-Dong
AU  - Lee SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - J Gastroenterol Hepatol
JT  - Journal of gastroenterology and hepatology
JID - 8607909
RN  - 0 (Endothelin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptor, Endothelin A)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Endothelin-1/*physiology
MH  - Gene Expression Regulation
MH  - Hypertension, Portal/*genetics/*physiopathology
MH  - Kidney/*blood supply
MH  - Lipopolysaccharides/*pharmacology
MH  - MAP Kinase Signaling System/genetics/physiology
MH  - Male
MH  - Rats, Sprague-Dawley
MH  - Receptor, Endothelin A/*genetics/*physiology
MH  - Up-Regulation
MH  - Vasoconstriction/drug effects/*genetics/*physiology
OTO - NOTNLM
OT  - endothelin receptor type A
OT  - lipopolysaccharide
OT  - partial portal vein ligation
OT  - portal hypertension
OT  - renal vascular reactivity
EDAT- 2014/07/06 06:00
MHDA- 2015/09/12 06:00
CRDT- 2014/07/04 06:00
PHST- 2014/05/26 00:00 [accepted]
PHST- 2014/07/04 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
AID - 10.1111/jgh.12670 [doi]
PST - ppublish
SO  - J Gastroenterol Hepatol. 2015 Jan;30(1):199-207. doi: 10.1111/jgh.12670.