PMID- 24989660
OWN - NLM
STAT- MEDLINE
DCOM- 20150516
LR  - 20181202
IS  - 1009-2587 (Print)
IS  - 1009-2587 (Linking)
VI  - 30
IP  - 2
DP  - 2014 Apr
TI  - [Effects of endotoxin/lipopolysaccharide on proliferation and apoptosis of human 
      umbilical cord mesenchymal stem cells].
PG  - 148-52
AB  - OBJECTIVE: To investigate the effects of different concentrations of 
      lipopolysaccharide (LPS) on proliferation and apoptosis of human umbilical cord 
      mesenchymal stem cells (hUCMSCs) in vitro, and to explore their possible 
      mechanism. METHODS: hUCMSCs from umbilical cord tissue of full-term healthy fetus 
      delivered by caesarean section were isolated and cultured in vitro using tissue 
      attachment method. The 3rd passage hUCMSCs were used in the study. Cells were 
      divided into groups A, B, C, D, and E, which were treated with DMEM/F12 medium 
      containing 0, 0.1, 1.0, 10.0, and 100.0 microg/mL of LPS respectively. In groups B, 
      C, D, and E, methyl-thiazole-tetrazolium assay was used to detect proliferative 
      activity of hUCMSCs at post treatment hour (PTH) 12, 24, and 48 (denoted as 
      absorption value), with 5 samples in each group at each time point; apoptosis of 
      hUCMSCs at PBH 24 was identified with acridine orange-ethidium bromide (AO-EB) 
      staining, with 4 samples in each group; apoptotic rate of hUCMSCs was determined 
      by flow cytometer, with 5 samples in each group. Above-mentioned indexes were 
      determined in group A at the same time points. Data were processed with analysis 
      of variance and LSD- t test. RESULTS: (1) There was no statistically significant 
      difference in proliferative activity of hUCMSCs at PTH 12 among groups A, B, C, 
      D, and E (with t values from -1.67 to 1.33, P values above 0.05). Compared with 
      that of group A, proliferative activity of hUCMSCs was increased in groups B, C, 
      and D at PTH 24 and 48 (with t values from -13.42 to 17.34, P < 0.05 or P < 
      0.01), especially so in group C. Proliferative activity of hUCMSCs was lower in 
      group E at PTH 24 and 48 than in group A (with t values respectively 8.64 and 
      17.34, P values below 0.01). (2) Obvious apoptosis of hUCMSCs was observed in 
      group E but not in the other 4 groups with AO-EB staining. (3) Apoptosis rates of 
      hUCMSCs in groups A, B, C, D, and E were respectively (3.1 +/- 0.6)%, (2.6 +/- 0.7)%, 
      (2.9 +/- 0.8)%, (3.1 +/- 0.4)%, (25.1 +/- 2.7)% (F = 272.19, P < 0.01). Apoptotic rate 
      of hUCMSCs in group B, C, or D was respectively close to that in group A (with t 
      values respectively 1.22, 0.57, -0.14, P values above 0.05), but it was higher in 
      group E than in group A (t = -17.63, P < 0.01). CONCLUSIONS: hUCMSCs 
      proliferation may be promoted by low concentration of LPS. hUCMSCs proliferation 
      is inhibited or induced to apoptosis along with the increase in concentration of 
      LPS, and it may be related to activation of different major molecular signaling 
      pathways by different concentrations of LPS.
FAU - Hou, Yusen
AU  - Hou Y
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China.
FAU - Chai, Jiake
AU  - Chai J
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China. Email: cjk304@126.
FAU - Liu, Lingying
AU  - Liu L
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China.
FAU - Duan, Hongjie
AU  - Duan H
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China.
FAU - Yu, Yonghui
AU  - Yu Y
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China.
FAU - Hu, Quan
AU  - Hu Q
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China.
FAU - Chu, Wanli
AU  - Chu W
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China.
FAU - Wang, Yihe
AU  - Wang Y
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China.
FAU - Luo, Hongmin
AU  - Luo H
AD  - Burns Institute, the First Hospital Affiliated to the PLA General Hospital, 
      Beijing 100048, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Shao Shang Za Zhi
JT  - Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
JID - 100959418
RN  - 0 (Endotoxins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (endotoxin binding proteins)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - *Cell Proliferation
MH  - Endotoxins/*adverse effects
MH  - Humans
MH  - Lipopolysaccharides/*pharmacology
MH  - Membrane Proteins
MH  - Mesenchymal Stem Cells/cytology/*drug effects
MH  - Signal Transduction
MH  - Umbilical Cord/cytology
EDAT- 2014/07/06 06:00
MHDA- 2015/05/20 06:00
CRDT- 2014/07/04 06:00
PHST- 2014/07/04 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PST - ppublish
SO  - Zhonghua Shao Shang Za Zhi. 2014 Apr;30(2):148-52.