PMID- 24991692
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140804
IS  - 1873-3344 (Electronic)
IS  - 0162-0134 (Linking)
VI  - 139
DP  - 2014 Oct
TI  - A monofunctional trinuclear platinum complex with steric hindrance demonstrates 
      strong cytotoxicity against tumor cells.
PG  - 77-84
LID - S0162-0134(14)00167-6 [pii]
LID - 10.1016/j.jinorgbio.2014.06.006 [doi]
AB  - Polynuclear platinum complexes constitute a special class of hopeful antitumor 
      agents. In this study, a Y-type monofunctional trinuclear platinum complex (MTPC) 
      with 1,3,5-tris(pyridin-2-ylmethoxy)benzene, ammine and chloride as ligands was 
      synthesized and characterized by (1)H NMR and electrospray ionization mass 
      spectrometry (ESI-MS). The DNA binding mode of MTPC was investigated using 
      circular dichroism spectroscopy and gel electrophoresis, and the reactivity of 
      MTPC towards glutathione was studied by (1)H NMR and ESI-MS. The results show 
      that MTPC can affect the conformation of calf-thymus DNA (CT-DNA) significantly 
      and tends to form 1,4-GG rather than 1,2-GG intrastrand crosslinks, which are 
      different from the instance of cisplatin. MTPC reacts with glutathione quite 
      slowly in comparison with cisplatin because of the steric hindrance. The 
      cytotoxicity of MTPC was tested on the human breast cancer cell line MCF-7, the 
      human non-small-cell lung cancer cell line A549, and the human ovarian cancer 
      cell line Skov-3 by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium 
      bromide] assay. MTPC is more potent than or comparable to cisplatin. The cellular 
      inhibition mode of MTPC was examined by flow cytometry using MCF-7 cells. MTPC 
      arrests the cell cycle mainly in G2 or M phase, while cisplatin arrests the cell 
      cycle in S phase. Similar to cisplatin, MTPC kills the cells predominantly 
      through an apoptotic pathway.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Wu, Shangnong
AU  - Wu S
AD  - State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical 
      Engineering, Nanjing University, Nanjing 210093, PR China.
FAU - Wang, Xiaoyong
AU  - Wang X
AD  - State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, 
      Nanjing University, Nanjing 210093, PR China; State Key Laboratory of Analytical 
      Chemistry for Life Science, Nanjing University, Nanjing 210093, PR China. 
      Electronic address: boxwxy@nju.edu.cn.
FAU - He, Yafeng
AU  - He Y
AD  - State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical 
      Engineering, Nanjing University, Nanjing 210093, PR China.
FAU - Zhu, Zhenzhu
AU  - Zhu Z
AD  - State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical 
      Engineering, Nanjing University, Nanjing 210093, PR China.
FAU - Zhu, Chengcheng
AU  - Zhu C
AD  - State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical 
      Engineering, Nanjing University, Nanjing 210093, PR China.
FAU - Guo, Zijian
AU  - Guo Z
AD  - State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical 
      Engineering, Nanjing University, Nanjing 210093, PR China. Electronic address: 
      zguo@nju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140617
PL  - United States
TA  - J Inorg Biochem
JT  - Journal of inorganic biochemistry
JID - 7905788
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Coordination Complexes)
RN  - 0 (Platinum Compounds)
RN  - 49DFR088MY (Platinum)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Antineoplastic Agents/chemistry/*pharmacology
MH  - Apoptosis
MH  - Base Sequence
MH  - Cell Cycle Checkpoints
MH  - Coordination Complexes/chemistry/*pharmacology
MH  - DNA/chemistry
MH  - Glutathione/chemistry
MH  - Humans
MH  - MCF-7 Cells
MH  - Platinum/chemistry
MH  - Platinum Compounds/chemistry/*pharmacology
OTO - NOTNLM
OT  - Antitumor drug
OT  - Apoptosis
OT  - DNA binding
OT  - Glutathione
OT  - Polynuclear platinum complex
EDAT- 2014/07/06 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/07/04 06:00
PHST- 2014/03/17 00:00 [received]
PHST- 2014/06/05 00:00 [revised]
PHST- 2014/06/08 00:00 [accepted]
PHST- 2014/07/04 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - S0162-0134(14)00167-6 [pii]
AID - 10.1016/j.jinorgbio.2014.06.006 [doi]
PST - ppublish
SO  - J Inorg Biochem. 2014 Oct;139:77-84. doi: 10.1016/j.jinorgbio.2014.06.006. Epub 
      2014 Jun 17.