PMID- 24991849
OWN - NLM
STAT- MEDLINE
DCOM- 20141002
LR  - 20140704
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 109
IP  - 3
DP  - 2014 Jul 3
TI  - New Zealand juvenile oyster mortality associated with ostreid herpesvirus 1-an 
      opportunistic longitudinal study.
PG  - 231-9
LID - 10.3354/dao02735 [doi]
AB  - During the 2010-11 summer outbreak of ostreid herpesvirus 1 (OsHV-1) in New 
      Zealand, an opportunistic longitudinal field study was conducted. OsHV-1 
      PCR-negative oyster spat (Crassostrea gigas) were relocated to an OsHV-1 
      PCR-positive area of the North Island of New Zealand that was experiencing 
      juvenile oyster mortalities. Over a period of 13 d, spat were monitored for 
      mortality, sampled for histopathology, and tested for the presence of OsHV-1 
      using real time PCR and Vibrio culture. Histopathology showed some evidence of 
      tissue pathology; however, no consistent progressive pathology was apparent. 
      Field mortalities were evident from Day 6 on. After 5 and 7 d of exposure, 83 and 
      100% of spat, respectively, tested positive for the virus by real time PCR. 
      Vibrio species recovered during the longitudinal study included V. splendidus and 
      V. aestuarianus. This study offers insight into the rapidity of onset and 
      virulence of the virus in naive oyster spat in New Zealand waters.
FAU - Keeling, S E
AU  - Keeling SE
AD  - Animal Health Laboratory, Investigation and Diagnostic Centre-Wallaceville, 
      Ministry for Primary Industries, 66 Ward St, PO Box 40742, Upper Hutt 5018, New 
      Zealand.
FAU - Brosnahan, C L
AU  - Brosnahan CL
FAU - Williams, R
AU  - Williams R
FAU - Gias, E
AU  - Gias E
FAU - Hannah, M
AU  - Hannah M
FAU - Bueno, R
AU  - Bueno R
FAU - McDonald, W L
AU  - McDonald WL
FAU - Johnston, C
AU  - Johnston C
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Crassostrea/*virology
MH  - DNA, Viral/genetics
MH  - Gene Expression Regulation, Viral
MH  - Herpesviridae/*classification/isolation & purification
MH  - Host-Pathogen Interactions
MH  - Sequence Alignment
MH  - Time Factors
EDAT- 2014/07/06 06:00
MHDA- 2014/10/03 06:00
CRDT- 2014/07/04 06:00
PHST- 2014/07/04 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2014/10/03 06:00 [medline]
AID - 10.3354/dao02735 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2014 Jul 3;109(3):231-9. doi: 10.3354/dao02735.