PMID- 24992030
OWN - NLM
STAT- MEDLINE
DCOM- 20140925
LR  - 20140721
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 547
IP  - 2
DP  - 2014 Sep 1
TI  - Dynamic expression of microRNAs in M2b polarized macrophages associated with 
      systemic lupus erythematosus.
PG  - 300-9
LID - S0378-1119(14)00745-8 [pii]
LID - 10.1016/j.gene.2014.06.065 [doi]
AB  - Macrophage polarization contributes to the initiation and perpetuation of 
      systemic lupus erythematosus (SLE). Our previous study demonstrated that M2b 
      polarized macrophages induced by activated lymphocyte-derived DNA (ALD-DNA) have 
      a crucial function in the initiation and progress of SLE disease. Accumulated 
      data suggest that microRNAs (miRNAs) serve as critical regulators to control 
      macrophage polarization. To investigate miRNA regulation during macrophage M2b 
      polarization of SLE, miRNA microarrays of murine bone marrow derived macrophages 
      (BMDMs) were performed following stimulation with ALD-DNA for 6 and 36 h. Over 
      11% of the 1111 analyzed miRNAs appeared differentially expressed during ALD-DNA 
      triggered macrophage M2b polarization. Cluster analysis revealed certain patterns 
      in miRNA expression that are closely linked to ALD-DNA induced macrophage M2b 
      polarization. Analysis of the network structure showed that the predicted 
      functions of the differentially regulated miRNAs at 6h are significantly 
      associated with inflammatory response and disease. Differentially regulated 
      miRNAs identified at 36 h were determined to be significantly related to cell 
      proliferation by biological network analysis. In this study, dynamic miRNA 
      expression patterns and network analysis are described for the first time during 
      ALD-DNA induced macrophage M2b polarization. The data not only provide a better 
      understanding of miRNA-mediated macrophage polarization but also demonstrate the 
      future therapeutic potential of targeting miRNAs in SLE patients.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Xiao, Pengli
AU  - Xiao P
AD  - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and 
      Medical Sciences, Soochow University, Suzhou, PR China.
FAU - Dong, Chunsheng
AU  - Dong C
AD  - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and 
      Medical Sciences, Soochow University, Suzhou, PR China.
FAU - Yue, Yan
AU  - Yue Y
AD  - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and 
      Medical Sciences, Soochow University, Suzhou, PR China.
FAU - Xiong, Sidong
AU  - Xiong S
AD  - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and 
      Medical Sciences, Soochow University, Suzhou, PR China. Electronic address: 
      sdxiongfd@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140630
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Cytokines/genetics/metabolism
MH  - DNA/pharmacology
MH  - Lupus Erythematosus, Systemic/*metabolism
MH  - Lymphocyte Activation
MH  - Lymphocytes/chemistry/immunology
MH  - *Macrophage Activation
MH  - Macrophages/drug effects/immunology/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/*metabolism
OTO - NOTNLM
OT  - ALD-DNA
OT  - M2b
OT  - Macrophage
OT  - MicroRNA
OT  - SLE
EDAT- 2014/07/06 06:00
MHDA- 2014/09/26 06:00
CRDT- 2014/07/04 06:00
PHST- 2014/03/30 00:00 [received]
PHST- 2014/06/12 00:00 [revised]
PHST- 2014/06/27 00:00 [accepted]
PHST- 2014/07/04 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2014/09/26 06:00 [medline]
AID - S0378-1119(14)00745-8 [pii]
AID - 10.1016/j.gene.2014.06.065 [doi]
PST - ppublish
SO  - Gene. 2014 Sep 1;547(2):300-9. doi: 10.1016/j.gene.2014.06.065. Epub 2014 Jun 30.