PMID- 24992828
OWN - NLM
STAT- MEDLINE
DCOM- 20150114
LR  - 20140716
IS  - 1744-8387 (Electronic)
IS  - 1478-9450 (Linking)
VI  - 11
IP  - 4
DP  - 2014 Aug
TI  - How proteomic ApoE serotyping could impact Alzheimer's disease risk assessment: 
      genetic testing by proteomics.
PG  - 405-7
LID - 10.1586/14789450.2014.936390 [doi]
AB  - Humans have three major apolipoprotein E (ApoE) alleles (APOE; epsilon2, epsilon3 and epsilon4) 
      that produce three ApoE protein isoforms. The epsilon2 allele encodes the ApoE2 isoform 
      (Cys112, Cys158), whereas epsilon3 encodes the wild-type ApoE3 isoform (Cys112, Arg158) 
      and epsilon4 encodes the ApoE4 isoform (Arg112, Arg158). Because the type of ApoE 
      expressed is related to sporadic Alzheimer's disease risk and familial 
      hyperlipidemia, many clinical studies have utilized ApoE typing in recent years. 
      ApoE serotyping is based on the correlation between ApoE genotype and isoform; it 
      is therefore possible to determine the genotype from the blood ApoE isoform 
      combination. Serotyping ApoE using mass spectrometry promises highly accurate 
      results while requiring minimal amounts of blood and reagents, resulting in lower 
      costs, which suggest that proteomic-based ApoE serotyping may eventually become a 
      routine clinical laboratory test. Not limited to ApoE, proteomic analysis of 
      human samples could be used to intentionally determine - and perhaps 
      unintentionally reveal - personal genetic information.
FAU - Nishimura, Motoi
AU  - Nishimura M
AD  - Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 
      Chiba, Japan.
FAU - Satoh, Mamoru
AU  - Satoh M
FAU - Matsushita, Kazuyuki
AU  - Matsushita K
FAU - Nomura, Fumio
AU  - Nomura F
LA  - eng
PT  - Editorial
PT  - Research Support, Non-U.S. Gov't
DEP - 20140704
PL  - England
TA  - Expert Rev Proteomics
JT  - Expert review of proteomics
JID - 101223548
RN  - 0 (Apolipoproteins E)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Alzheimer Disease/*diagnosis
MH  - Apolipoproteins E/*blood/classification
MH  - Genetic Testing
MH  - Humans
MH  - Hyperlipidemias/diagnosis
MH  - Molecular Diagnostic Techniques/economics/*methods
MH  - Protein Isoforms
MH  - Proteomics/economics/*methods
OTO - NOTNLM
OT  - APOEepsilon2 allele
OT  - APOEepsilon4 allele
OT  - ApoE2 isoform
OT  - ApoE4 isoform
OT  - apolipoprotein E
OT  - dementia risk
OT  - familial hyperlipidemia
OT  - genetic testing
OT  - proteomics
OT  - sporadic Alzheimer disease
EDAT- 2014/07/06 06:00
MHDA- 2015/01/15 06:00
CRDT- 2014/07/05 06:00
PHST- 2014/07/05 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/01/15 06:00 [medline]
AID - 10.1586/14789450.2014.936390 [doi]
PST - ppublish
SO  - Expert Rev Proteomics. 2014 Aug;11(4):405-7. doi: 10.1586/14789450.2014.936390. 
      Epub 2014 Jul 4.